| A novel application of the buccal micronucleus cytome assay in oral lichen planus: A pilot study. | |
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MedLine Citation:
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PMID: 21429474 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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BACKGROUND: The most important complication of oral lichen planus is malignancy transformation. OBJECTIVE: The aim of this study was to assess cellular and nuclear morphology in a group of patients with oral lichen planus measured by means of buccal micronucleus cytome assay. STUDY DESIGN: This study included thirty patients with a clinicopathological diagnosis of oral lichen planus (all with atrophic-erosive clinical forms of OLP) and thirty healthy control subjects. Both samples were similar in age and gender. The buccal micronucleus cytome assay protocol consisted of: cell collection from both cheeks with a cytobrush; cell centrifuge; slide preparation, fixation and staining followed by fluorescent microscope analysis. 2×10(6) exfoliated cells were screened for nuclear abnormalities: micronuclei, nuclear buds, binucleation, basal and differentiated cells, condensed chromatin, karyorrhectic cells, pyknosis and karyolytic cells. RESULTS: Patients with oral lichen planus showed significantly higher frequencies of micronuclei (p<0.001), nuclear buds (p<0.001), binucleated cells (p<0.021) than the control group. CONCLUSIONS: This method is an easy way for clinicians to assess DNA damage, proliferative potential of basal cells and cell death in buccal cells in cases of oral lichen planus. |
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Authors:
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M Sanchez-Siles; I Ros-Llor; F Camacho-Alonso; P Lopez-Jornet |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-21 |
Journal Detail:
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Title: Archives of oral biology Volume: - ISSN: 1879-1506 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0116711 Medline TA: Arch Oral Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Ltd. All rights reserved. |
Affiliation:
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Oral Medicine Department, Morales Meseguer Hospital, University of Murcia, Spain. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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