Document Detail


A novel antioestrogen agent (3R,6R)-bassiatin inhibits cell proliferation and cell cycle progression by repressing cyclin D1 expression in 17β-oestradiol-treated MCF-7 cells.
MedLine Citation:
PMID:  21241249     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bassiatin [(3R,6R)-bassiatin] was isolated from the endogenous fungus Fusarium oxysporum J8-1-2. We found that oestrogen-dependent cell growth was inhibited by bassiatin in MCF-7 ERα (oestrogen receptor α)-positive breast cancer cells. In addition, the mRNA and protein levels of ERα and the oestrogen-responsive gene cyclin D1 were down-regulated by bassiatin in the presence of 17β-oestradiol. Co-treatment of 17β-oestradiol and bassiatin increased phospho-cyclin D1 (Thr286), an indicator of cyclin D1 phosphorylation-dependent degradation. However, this effect was not obvious in the absence of 17β-oestradiol, suggesting that bassiatin may play a role in the metabolism of cyclin D1 by decreasing cyclin D1 protein expression in the presence of 17β-oestradiol. Cyclin D1, known as a key cell cycle regulator, regulates the transition of G1- and S-phase. Decreased cyclin D1 was found to be involved in bassiatin-induced MCF-7 cell cycle arrest. Collectively, our study showed that bassiatin induced cell cycle arrest and exerted an antioestrogen effect. It may prove to be a potential drug target for antioestrogen therapy in ERα-positive breast cancer.
Authors:
Li Meng; Bo Feng; Hongwen Tao; Tingting Yang; Yan Meng; Weiming Zhu; Caiguo Huang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  35     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-05-12     Completed Date:  2011-09-02     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  599-605     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, Peoples Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cell Proliferation / drug effects
Cyclin D1 / genetics,  metabolism*
Down-Regulation
Estradiol / pharmacology*
Estrogen Receptor Modulators / chemistry,  pharmacology*
Estrogen Receptor alpha / chemistry,  genetics,  metabolism*
Fusarium / chemistry
G1 Phase / drug effects
Humans
Morpholines / chemistry,  pharmacology*
Phosphorylation
S Phase / drug effects
Stereoisomerism
Chemical
Reg. No./Substance:
0/Estrogen Receptor Modulators; 0/Estrogen Receptor alpha; 0/Morpholines; 136601-57-5/Cyclin D1; 50-28-2/Estradiol; 65454-13-9/lateritin

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