Document Detail


A novel amplification target, ARHGAP5, promotes cell spreading and migration by negatively regulating RhoA in Huh-7 hepatocellular carcinoma cells.
MedLine Citation:
PMID:  18996642     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
RhoA, a member of the Rho family of small GTPases, directs the organization of the actin cytoskeleton and is involved in regulating cell shape and movement. Its activity is negatively regulated by p190-B RhoGAP (GTPase-activating protein). We investigated DNA copy number aberrations in human hepatocellular carcinoma and esophageal squamous cell carcinoma cell lines using a high-density oligonucleotide microarray and found a novel amplification at chromosomal region 14q12. We identified ARHGAP5 (the gene encoding p190-B RhoGAP) as a probable target for the amplification at 14q12, and our results showed that p190-B RhoGAP promotes cells spreading and migration by negatively regulating RhoA activity in Huh-7 hepatocellular carcinoma cells.
Authors:
Yasuyuki Gen; Kohichiroh Yasui; Keika Zen; Tomoaki Nakajima; Kazuhiro Tsuji; Mio Endo; Hironori Mitsuyoshi; Masahito Minami; Yoshito Itoh; Shinji Tanaka; Masafumi Taniwaki; Shigeki Arii; Takeshi Okanoue; Toshikazu Yoshikawa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-08
Journal Detail:
Title:  Cancer letters     Volume:  275     ISSN:  1872-7980     ISO Abbreviation:  Cancer Lett.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-01-28     Completed Date:  2009-02-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  27-34     Citation Subset:  IM    
Affiliation:
Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, Kyoto 602-8566, Japan.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Hepatocellular / metabolism*
Cell Line, Tumor
Cell Movement
Chromosomes, Human, Pair 14 / ultrastructure
Cytoskeleton / metabolism
DNA / metabolism
Disease Progression
GTPase-Activating Proteins / metabolism*
Gene Expression Regulation, Neoplastic*
Humans
In Situ Hybridization, Fluorescence
Liver Neoplasms / metabolism*
RNA Interference
Reverse Transcriptase Polymerase Chain Reaction
rhoA GTP-Binding Protein / metabolism*
Chemical
Reg. No./Substance:
0/ARHGAP5 protein, human; 0/GTPase-Activating Proteins; 124671-05-2/RHOA protein, human; 9007-49-2/DNA; EC 3.6.5.2/rhoA GTP-Binding Protein

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