Document Detail


A novel amino acid supplementation strategy based on a stoichiometric model to enhance human IL-2 (interleukin-2) expression in high-cell-density Escherichia coli cultures.
MedLine Citation:
PMID:  21062264     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
A novel amino acid supplementation strategy was developed for enhancing the production of IL-2 (interleukin-2; as a model protein) by recombinant Escherichia coli BL21 (pET21a-hil2) in fed-batch high-cell-density cultures. The amino acids most needed and their amounts were determined using a stoichiometric model, and full factorial design experiments were conducted to determine the effects of single amino acids and amino acid mixtures on production. One of the most effective amino acid mixtures was found to be leucine, aspartic acid and glycine. This amino acid mixture was utilized for the production of IL-2 in batch and fed-batch fermentations. The amount of IL-2 produced increased from 403 to 722 mg/l and from 5.15 × 10³ to 8.08 × 10³ mg/l in batch and fed-batch cultures respectively. The results also revealed that the above amino acid mixture specifically increases IL-2 concentration in the cells.
Authors:
Shahin Yegane Sarkandy; Rasoul Khalilzadeh; Seyed Abbas Shojaosadati; Majid Sadeghizadeh; Amir Mohammad Farnoud; Valiollah Babaeipour; Amir Maghsoudi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biotechnology and applied biochemistry     Volume:  57     ISSN:  1470-8744     ISO Abbreviation:  Biotechnol. Appl. Biochem.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-01-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8609465     Medline TA:  Biotechnol Appl Biochem     Country:  England    
Other Details:
Languages:  eng     Pagination:  151-6     Citation Subset:  IM    
Affiliation:
Biotechnology Group, Chemical Engineering Department, Faculty of Engineering, Tarbiat Modares University, PO Box 14115-143, Tehran, Iran.
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