| A novel adipokine GM2AP impairs insulin signaling. | |
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MedLine Citation:
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PMID: 21036149 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In an attempt to discover novel adipokines, we performed proteomics analyses using culture medium from differentiated 3T3-L1 adipocytes, and first identified GM2AP. The levels of GM2AP mRNA and protein were augmented by adipogenesis in cultured adipocytes and expression in adipose tissue and serum of obese mice or human subjects was found to be significantly higher than in lean counterparts. Exposure of 3T3-L1 adipocytes to GM2AP protein accelerated dissociation of insulin receptor-beta (IRβ) from caveolin-1, and interrupted insulin signal transduction. Abrogation of GM2AP function by specific antibodies augmented glucose uptake. Furthermore, treatment of rat pheochromocytoma PC12 NS1 cells with GM2AP impaired NGF signal transduction. Taken together, these results provide novel insights into the physiological functions of GM2AP in obesity. |
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Authors:
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Kiyoshi Higashi; Toshiyuki Mikami; Toru Yamada; Hitoshi Kawashima; Toru Kimura; Hideo Kaneko |
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Publication Detail:
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Type: Journal Article Date: 2010-10-29 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 402 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-24 Completed Date: 2010-12-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 571-6 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Environmental Health Science Laboratory, Sumitomo Chemical Co. Ltd., Oaka 554-8558, Japan. higashik2@sc.sumitomo-chem.co.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3-L1 Cells Adipocytes / drug effects, metabolism, physiology* Adipogenesis* Adipokines / metabolism* Animals Cells, Cultured G(M2) Activator Protein / metabolism* Humans Insulin / metabolism*, pharmacology Mice Obesity / genetics, metabolism* Proteomics Rats |
| Chemical | |
Reg. No./Substance:
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0/Adipokines; 0/G(M2) Activator Protein; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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