| A novel T cell activation antigen identified by monoclonal IMN3.1 antibody and expressed preferentially on human T cells susceptible to apoptotic cell death. | |
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MedLine Citation:
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PMID: 7682236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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When cultured without appropriate growth factors, most of activated (CD45RO+) T cells expanded in acute EBV-induced infectious mononucleosis (IM) easily die via an apoptotic cell death mechanism, indicating one Ag-driven selection in the periphery. In this work, we attempted to raise the mAb against cell surface molecules, preferentially expressed on T cells entering apoptosis, by immunizing PBMC from an acute IM patient. We obtained one mAb, termed IMN3.1, that could define clearly the expansion of activated (CD45RO+) T cells in the blood of acute IM patients. In contrast to its intense expression on IM T cells, the IMN3.1-reactive Ag was only dimly expressed on CD45RO+ (memory or previously activated) populations of T cells from normal individuals. Although naive (CD45RO-) populations of T cells usually lacked IMN3.1 Ag, this Ag was inducible relatively late after in vitro activation of naive T cells. The cellular distribution and molecular characterization of IMN3.1-reactive Ag suggested that IMN3.1 mAb appeared to recognize a novel activation-associated cell surface determinant of about 120 kDa m.w., which might be predominantly expressed on apoptosis-prone T cell lineage cells, such as IM T cells, thymocytes, cytokine-dependent T cell lines, and anti-Fas-sensitive T cell lines. |
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Authors:
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T Uehara; T Miyawaki; S Natsuume-Sakai; R Nibu; M Hasui; A Yachie; S Shimizu; N Taniguchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 150 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1993 Apr |
Date Detail:
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Created Date: 1993-05-12 Completed Date: 1993-05-12 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3243-53 Citation Subset: AIM; IM |
Affiliation:
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Department of Pediatrics, School of Medicine, Kanazawa University, Ishikawa, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Animals Antibodies, Monoclonal / biosynthesis, immunology* Antigens, CD27 Antigens, CD45 / analysis Antigens, CD95 Antigens, Differentiation, T-Lymphocyte / analysis*, immunology Antigens, Surface / analysis, immunology Apoptosis* Cell Line Child Fluorescent Antibody Technique Humans Infant, Newborn Lymphocyte Activation* Mice Mice, Inbred BALB C T-Lymphocytes / immunology*, physiology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, CD27; 0/Antigens, CD95; 0/Antigens, Differentiation, T-Lymphocyte; 0/Antigens, Surface; EC 3.1.3.48/Antigens, CD45 |
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