Document Detail


A novel RNA silencing vector to improve antigen expression and stability in Chinese hamster ovary cells.
MedLine Citation:
PMID:  17428585     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chinese hamster ovary (CHO) cells and dihydrofolate reductase (dhfr)/methotrexate (MTX) gene amplification system are routinely used to generate stable producer CHO cell clones in biopharmaceutical industries. The present study proposes a novel method by the co-amplification of the silencing vector targeted to dhfr gene for improvements of selecting high-producing clones in dhfr-deficient and wild-type CHO cells. Using the silencing vector also resulted in improving the stability of the recombinant protein expression in the absence of MTX in the CHO/dhFr(-) and wild-type CHO cells. This new method is proposed to generate highly expressed stable cell clones of both dhfr-deficient and wild-type CHO cells for recombinant antigen production. Utilization of the silencing vector designed in this study can improve antigen expression through dhfr-directed gene amplification in other dhfr-competent cell lines for vaccine development.
Authors:
Willy W L Hong; Suh-Chin Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-02-26
Journal Detail:
Title:  Vaccine     Volume:  25     ISSN:  0264-410X     ISO Abbreviation:  Vaccine     Publication Date:  2007 May 
Date Detail:
Created Date:  2007-04-27     Completed Date:  2007-10-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8406899     Medline TA:  Vaccine     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  4103-11     Citation Subset:  IM    
Affiliation:
Institute of Biotechnology, Department of Life Science, National Tsing-Hua University, Hsinchu 30013, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens / biosynthesis*,  genetics
Base Sequence
CHO Cells
Clone Cells
Cricetinae
Cricetulus
Gene Amplification
Genetic Vectors / genetics*
Green Fluorescent Proteins
Methotrexate / pharmacology
Mice
Molecular Sequence Data
RNA Interference*
Tetrahydrofolate Dehydrogenase / biosynthesis,  deficiency,  genetics*
Transfection
Chemical
Reg. No./Substance:
0/Antigens; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 59-05-2/Methotrexate; EC 1.5.1.3/Tetrahydrofolate Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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