| The novel NhaE-type Na(+)/H (+) antiporter of the pathogenic bacterium Neisseria meningitidis. | |
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MedLine Citation:
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PMID: 23208205 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Neisseria meningitidis is a pathogenic bacterium responsible for meningitis. The mechanisms underlying the control of Na(+) transmembrane movement, presumably important to pathogenicity, have been barely addressed. To elucidate the function of the components of the Na(+) transport system in N. meningitidis, an open reading frame from the genome of this bacterium displaying similarity with the NhaE type of Na(+)/H(+) antiporters was expressed in Escherichia coli and characterized for sodium transport ability. The N. meningitidis antiporter (NmNhaE) was able to complement an E. coli strain devoid of Na(+)/H(+) antiporters (KNabc) respecting the ability to grow in the presence of NaCl and LiCl. Ion transport assays in everted vesicles prepared from KNabc expressing NmNhaE from a plasmid confirmed its ability to translocate Na(+) and Li(+). Here is presented the characterization of the first NhaE from a pathogen, an important contribution to the comprehension of sodium ion metabolism in this kind of microorganisms. |
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Authors:
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Pedro M F Sousa; Marco A M Videira; Thomas Vorburger; Sara T N Silva; James W Moir; Julia Steuber; Ana M P Melo |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-4 |
Journal Detail:
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Title: Archives of microbiology Volume: - ISSN: 1432-072X ISO Abbreviation: Arch. Microbiol. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2012-12-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0410427 Medline TA: Arch Microbiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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BioTrop, Instituto de Investigação Científica Tropical, R. da Junqueira, 86, 1300-344, Lisboa, Portugal. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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