Document Detail


The novel NhaE-type Na(+)/H (+) antiporter of the pathogenic bacterium Neisseria meningitidis.
MedLine Citation:
PMID:  23208205     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Neisseria meningitidis is a pathogenic bacterium responsible for meningitis. The mechanisms underlying the control of Na(+) transmembrane movement, presumably important to pathogenicity, have been barely addressed. To elucidate the function of the components of the Na(+) transport system in N. meningitidis, an open reading frame from the genome of this bacterium displaying similarity with the NhaE type of Na(+)/H(+) antiporters was expressed in Escherichia coli and characterized for sodium transport ability. The N. meningitidis antiporter (NmNhaE) was able to complement an E. coli strain devoid of Na(+)/H(+) antiporters (KNabc) respecting the ability to grow in the presence of NaCl and LiCl. Ion transport assays in everted vesicles prepared from KNabc expressing NmNhaE from a plasmid confirmed its ability to translocate Na(+) and Li(+). Here is presented the characterization of the first NhaE from a pathogen, an important contribution to the comprehension of sodium ion metabolism in this kind of microorganisms.
Authors:
Pedro M F Sousa; Marco A M Videira; Thomas Vorburger; Sara T N Silva; James W Moir; Julia Steuber; Ana M P Melo
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-12-4
Journal Detail:
Title:  Archives of microbiology     Volume:  -     ISSN:  1432-072X     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
BioTrop, Instituto de Investigação Científica Tropical, R. da Junqueira, 86, 1300-344, Lisboa, Portugal.
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