| A novel L: -isoleucine metabolism in Bacillus thuringiensis generating (2S,3R,4S)-4-hydroxyisoleucine, a potential insulinotropic and anti-obesity amino acid. | |
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MedLine Citation:
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PMID: 21069315 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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4-Hydroxyisoleucine (HIL) found in fenugreek seeds has insulinotropic and anti-obesity effects and is expected to be a novel orally active drug for insulin-independent diabetes. Here, we show that the newly isolated strain Bacillus thuringiensis 2e2 and the closely related strain B. thuringiensis ATCC 35646 operate a novel metabolic pathway for L: -isoleucine (L: -Ile) via HIL and 2-amino-3-methyl-4-ketopentanoic acid (AMKP). The HIL synthesis was catalyzed stereoselectively by an α-ketoglutaric acid-dependent dioxygenase and to be useful for efficient production of a naturally occurring HIL isomer, (2S,3R,4S)-HIL. The (2S,3R,4S)-HIL was oxidized to (2S,3R)-AMKP by a NAD(+)-dependent dehydrogenase. The metabolic pathway functions as an effective bypass pathway that compensates for the incomplete tricarboxylic acid (TCA) cycle in Bacillus species and also explains how AMKP, a vitamin B(12) antimetabolite with antibiotic activity, is synthesized. These novel findings pave a new way for the commercial production of HIL and also for AMKP. |
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Authors:
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Jun Ogawa; Tomohiro Kodera; Sergey V Smirnov; Makoto Hibi; Natalia N Samsonova; Ryoukichi Koyama; Hiroyuki Yamanaka; Junichi Mano; Takashi Kawashima; Kenzo Yokozeki; Sakayu Shimizu |
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Publication Detail:
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Type: Journal Article Date: 2010-11-11 |
Journal Detail:
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Title: Applied microbiology and biotechnology Volume: 89 ISSN: 1432-0614 ISO Abbreviation: Appl. Microbiol. Biotechnol. Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8406612 Medline TA: Appl Microbiol Biotechnol Country: Germany |
Other Details:
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Languages: eng Pagination: 1929-38 Citation Subset: IM |
Affiliation:
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Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto, 606-8502, Japan, ogawa@kais.kyoto-u.ac.jp. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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