Document Detail


A novel Drosophila Minute locus encodes ribosomal protein S13.
MedLine Citation:
PMID:  8725235     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Minutes comprise > 50 phenotypically similar Drosophila mutations believed to affect ribosomal protein genes. Common traits of the Minute phenotype are short and thin bristles, slow development, and recessive lethality. To further investigate the proposed Minute to ribosomal protein correspondence, loss-of-function Minute mutations were induced by P-element mutagenesis. Here, we report a previously undescribed Minute locus that maps to 32A on chromosome 2L; this Minute allele is named P{lacW}M(2)32A1 and the gene M(2)32A. Flies heterozygous for P{lacW}M(2)32A1 have a medium Minute phenotype. The gene interrupted by the P-element insertion was cloned. Sequence analyses revealed that it encodes the Drosophila homologue of eukaryotic ribosomal protein S13. It is a single-copy gene and the level of RPS13 transcript is reduced to approximately 50% in P(lacW) M(2)32A1 heterozygotes. Both transcript level and phenotype are restored to wild type by remobilizing the P-element, demonstrating that the mutation is caused by insertion of the P-element construct. These results further strengthen the notion that Minutes encode ribosomal proteins and demonstrate that P-element mutagenesis is a fruitful approach to use in these studies.
Authors:
S Saebøe-Larssen; A Lambertsson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genetics     Volume:  143     ISSN:  0016-6731     ISO Abbreviation:  Genetics     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1997-02-05     Completed Date:  1997-02-05     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0374636     Medline TA:  Genetics     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  877-85     Citation Subset:  IM    
Affiliation:
Department of Biology, University of Oslo, Norway.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/X91853;  X91854
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Chromosome Banding
Chromosome Mapping*
Drosophila / genetics*
Molecular Sequence Data
Mutagenesis
Ribosomal Proteins / genetics*
Sequence Alignment
Chemical
Reg. No./Substance:
0/Ribosomal Proteins
Comments/Corrections

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