| A novel Drosophila Minute locus encodes ribosomal protein S13. | |
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MedLine Citation:
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PMID: 8725235 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Minutes comprise > 50 phenotypically similar Drosophila mutations believed to affect ribosomal protein genes. Common traits of the Minute phenotype are short and thin bristles, slow development, and recessive lethality. To further investigate the proposed Minute to ribosomal protein correspondence, loss-of-function Minute mutations were induced by P-element mutagenesis. Here, we report a previously undescribed Minute locus that maps to 32A on chromosome 2L; this Minute allele is named P{lacW}M(2)32A1 and the gene M(2)32A. Flies heterozygous for P{lacW}M(2)32A1 have a medium Minute phenotype. The gene interrupted by the P-element insertion was cloned. Sequence analyses revealed that it encodes the Drosophila homologue of eukaryotic ribosomal protein S13. It is a single-copy gene and the level of RPS13 transcript is reduced to approximately 50% in P(lacW) M(2)32A1 heterozygotes. Both transcript level and phenotype are restored to wild type by remobilizing the P-element, demonstrating that the mutation is caused by insertion of the P-element construct. These results further strengthen the notion that Minutes encode ribosomal proteins and demonstrate that P-element mutagenesis is a fruitful approach to use in these studies. |
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Authors:
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S Saebøe-Larssen; A Lambertsson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Genetics Volume: 143 ISSN: 0016-6731 ISO Abbreviation: Genetics Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1997-02-05 Completed Date: 1997-02-05 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0374636 Medline TA: Genetics Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 877-85 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Oslo, Norway. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/X91853; X91854 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence Chromosome Banding Chromosome Mapping* Drosophila / genetics* Molecular Sequence Data Mutagenesis Ribosomal Proteins / genetics* Sequence Alignment |
| Chemical | |
Reg. No./Substance:
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0/Ribosomal Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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