| A novel Delta12-fatty acid desaturase gene from methylotrophic yeast Pichia pastoris GS115. | |
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MedLine Citation:
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PMID: 17063209 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The methylotrophic yeast Pichia pastoris GS115, a widely used strain in production of various heterologous proteins, especially membrane-bound enzymes, can also produce linoleic and linolenic acids, which indicates the existence of membrane-bound Delta12 and Delta15-fatty acid desaturases. This paper describes the cloning and functional characterization of a novel Delta12-fatty acid desaturase gene from this methylotrophic yeast. The open reading frame of the gene (named Pp-FAD12) is 1263 bp in size and encodes a 420-amino-acid peptide. The deduced Pp-FAD12 protein shows high identity (50-67%) with Delta12-fatty acid desaturases from other fungi. It also shows a high identity (57%) with Delta15-fatty acid desaturase (named Sk-FAD15) from Saccharomyces kluyveri. Expression of Pp-FAD12 in polyunsaturated fatty acids non-producing yeast Saccharomyces cerevisiae demonstrated that its product converted oleic acid (18 : 1) to linoleic acid (18 : 2). This result suggests that Pp-FAD12 encodes a novel Delta12-fatty acid desaturase in P. pastoris GS115. This is the first report about the cloning and functional characterization of Delta12-fatty acid desaturase gene in methylotrophic yeast. |
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Authors:
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D Sh Wei; M Ch Li; X X Zhang; H Zhou; L J Xing |
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Publication Detail:
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Type: Journal Article Date: 2006-10-26 |
Journal Detail:
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Title: Acta biochimica Polonica Volume: 53 ISSN: 0001-527X ISO Abbreviation: Acta Biochim. Pol. Publication Date: 2006 |
Date Detail:
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Created Date: 2007-01-03 Completed Date: 2007-10-17 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 14520300R Medline TA: Acta Biochim Pol Country: Poland |
Other Details:
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Languages: eng Pagination: 753-9 Citation Subset: IM |
Affiliation:
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Tianjin Key Laboratory of Microbial Functional Genomics, Department of Microbiology, Nankai University, Tianjin 300071, PR China. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AAX20125 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Fatty Acid Desaturases / genetics*, metabolism Genes, Fungal Linoleic Acid / metabolism Molecular Sequence Data Oleic Acid / metabolism Open Reading Frames Pichia / enzymology, genetics* Sequence Homology |
| Chemical | |
Reg. No./Substance:
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112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-12 fatty acid desaturase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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