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A novel Delta12-fatty acid desaturase gene from methylotrophic yeast Pichia pastoris GS115.
MedLine Citation:
PMID:  17063209     Owner:  NLM     Status:  MEDLINE    
The methylotrophic yeast Pichia pastoris GS115, a widely used strain in production of various heterologous proteins, especially membrane-bound enzymes, can also produce linoleic and linolenic acids, which indicates the existence of membrane-bound Delta12 and Delta15-fatty acid desaturases. This paper describes the cloning and functional characterization of a novel Delta12-fatty acid desaturase gene from this methylotrophic yeast. The open reading frame of the gene (named Pp-FAD12) is 1263 bp in size and encodes a 420-amino-acid peptide. The deduced Pp-FAD12 protein shows high identity (50-67%) with Delta12-fatty acid desaturases from other fungi. It also shows a high identity (57%) with Delta15-fatty acid desaturase (named Sk-FAD15) from Saccharomyces kluyveri. Expression of Pp-FAD12 in polyunsaturated fatty acids non-producing yeast Saccharomyces cerevisiae demonstrated that its product converted oleic acid (18 : 1) to linoleic acid (18 : 2). This result suggests that Pp-FAD12 encodes a novel Delta12-fatty acid desaturase in P. pastoris GS115. This is the first report about the cloning and functional characterization of Delta12-fatty acid desaturase gene in methylotrophic yeast.
D Sh Wei; M Ch Li; X X Zhang; H Zhou; L J Xing
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Publication Detail:
Type:  Journal Article     Date:  2006-10-26
Journal Detail:
Title:  Acta biochimica Polonica     Volume:  53     ISSN:  0001-527X     ISO Abbreviation:  Acta Biochim. Pol.     Publication Date:  2006  
Date Detail:
Created Date:  2007-01-03     Completed Date:  2007-10-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  14520300R     Medline TA:  Acta Biochim Pol     Country:  Poland    
Other Details:
Languages:  eng     Pagination:  753-9     Citation Subset:  IM    
Tianjin Key Laboratory of Microbial Functional Genomics, Department of Microbiology, Nankai University, Tianjin 300071, PR China.
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MeSH Terms
Base Sequence
Fatty Acid Desaturases / genetics*,  metabolism
Genes, Fungal
Linoleic Acid / metabolism
Molecular Sequence Data
Oleic Acid / metabolism
Open Reading Frames
Pichia / enzymology,  genetics*
Sequence Homology
Reg. No./Substance:
112-80-1/Oleic Acid; 2197-37-7/Linoleic Acid; EC 1.14.19.-/Fatty Acid Desaturases; EC 1.14.99.-/delta-12 fatty acid desaturase

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