Document Detail

A note concerning acetate activation of peroxidative activity of catalases using 2,2'-azino-bis(3-ethylbenzthiazoline)-6-sulfonic acid as a substrate.
MedLine Citation:
PMID:  15932252     Owner:  NLM     Status:  MEDLINE    
Beef liver catalases showed peroxidative activity using 2,2'-azino-bis-(3-ethylbenzthiazoline)-6-sulfonic acid as the electron donor and hydrogen peroxide as the acceptor at a pH of 5. This activity was not observed at pH 7. The reaction depended on acetate concentration, although succinate and propionate could partly replace the acetate as a catalyst. Other haem proteins also catalyzed a peroxidative effect. The reaction using syringaldazine or the coupling between dimethylaminobenzoic acid and 3-methyl-2-benzothiazolinone hydrazone was less effective and less sensitive. Evidence is presented that the reaction is associated with a conformational change of the catalase.
Warren L Baker; Christopher Key; Greg T Lonergan
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnology progress     Volume:  21     ISSN:  8756-7938     ISO Abbreviation:  Biotechnol. Prog.     Publication Date:    2005 May-Jun
Date Detail:
Created Date:  2005-06-03     Completed Date:  2005-09-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506292     Medline TA:  Biotechnol Prog     Country:  United States    
Other Details:
Languages:  eng     Pagination:  751-5     Citation Subset:  IM    
Environment and Biotechnology Centre, Swinburne University of Technology, John Street, Hawthorn, 3122, Melbourne, Australia.
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MeSH Terms
Catalase / chemistry*
Enzyme Activation
Enzyme Stability
Hydrogen Peroxide / chemistry*
Hydrogen-Ion Concentration
Liver / enzymology*
Substrate Specificity
Sulfonic Acids / chemistry*
Reg. No./Substance:
0/Benzothiazoles; 0/Sulfonic Acids; 28752-68-3/2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; 7722-84-1/Hydrogen Peroxide; EC

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