| The notch ligand Delta1 recruits Dlg1 at cell-cell contacts and regulates cell migration. | |
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MedLine Citation:
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PMID: 15485825 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Delta1 acts as a membrane-bound ligand that interacts with the Notch receptor and plays a critical role in cell fate specification. By using peptide affinity chromatography followed by mass spectrometry, we have identified Dlg1 as a partner of the Delta1 C-terminal region. Dlg1 is a human homolog of the Drosophila Discs large tumor suppressor, a member of the membrane-associated guanylate kinase family of molecular scaffolds. We confirmed this interaction by co-immunoprecipitation experiments between endogenous Dlg1 and transduced Delta1 in a 3T3 cell line stably expressing Delta1. Moreover, we showed that deletion of a canonical C-terminal PDZ-binding motif (ATEV) in Delta1 abrogated this interaction. Delta4 also interacted with Dlg1, whereas Jagged1, another Notch ligand, did not. In HeLa cells, transfected Delta1 triggered the accumulation of endogenous Dlg1 at sites of cell-cell contact. Expression of Delta1 also reduced the motility of 3T3 cells. Finally, deletion of the ATEV motif totally abolished these effects but did not interfere with the ability of Delta1 to induce Notch signaling and T cell differentiation in co-culture experiments. These results point to a new, probably cell-autonomous function of Delta1, which is independent of its activity as a Notch ligand. |
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Authors:
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Emmanuelle M Six; Delphine Ndiaye; Guido Sauer; Yacine Laâbi; Rafika Athman; Ana Cumano; Christel Brou; Alain Israël; Frédérique Logeat |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-10-14 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 279 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2004 Dec |
Date Detail:
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Created Date: 2004-12-23 Completed Date: 2005-02-25 Revised Date: 2011-09-13 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 55818-26 Citation Subset: IM |
Affiliation:
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Unité de Biologie Moléculaire de l'Expression Génique, URA 2582 CNRS, Institut Pasteur, 25 Rue du Dr Roux, 75724 Paris Cedex 15, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Adaptor Proteins, Signal Transducing Amino Acid Motifs Amino Acid Sequence Animals Biotinylation Cell Communication Cell Differentiation Cell Line Cell Movement Electrophoresis, Polyacrylamide Gel Flow Cytometry Green Fluorescent Proteins / metabolism Hela Cells Hematopoietic Stem Cells / metabolism Humans Immunoblotting Immunoprecipitation Ligands Mass Spectrometry Membrane Proteins / chemistry, metabolism, physiology* Mice Microscopy, Fluorescence Molecular Sequence Data Plasmids / metabolism Protein Binding Protein Structure, Tertiary Proteins / chemistry, physiology* Receptors, Notch Sequence Homology, Amino Acid Transfection Wound Healing |
| Chemical | |
Reg. No./Substance:
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0/Adaptor Proteins, Signal Transducing; 0/DLG1 protein, human; 0/Ligands; 0/Membrane Proteins; 0/Proteins; 0/Receptors, Notch; 0/delta protein; 147336-22-9/Green Fluorescent Proteins; EC 2.7.4.8/Dlgh1 protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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