Document Detail


The nonstop transvesical cystometrogram in urethane-anesthetized rats: a simple procedure for quantitative studies on the various phases of urinary bladder voiding cycle.
MedLine Citation:
PMID:  3702468     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new cystometric procedure that involves a nonstop transvesical (through a needle inserted into the bladder dome) infusion of saline in urethane-anesthetized rats is described. This permits the obtainment of a series of repetitive voiding cycles in both male and female rats. Recording at high-chart speed permits quantitative measurements of the various phases of the voiding cycle. Micturition was almost invariably associated with appearance, during a sustained increase of intravesical pressure, of a series of high-frequency oscillations, which were paralleled by a stream-like emission of the infused fluid. Micturition was not observed after topical tetrodotoxin, after bilateral transection of pelvic nerves, or in acute spinal rats. Intravenous hexamethonium produced a dose-related impairment of the voiding cycle, and, at high doses, suppressed micturition, and overflow incontinence ensued. The experimental procedure described herein appears to be suitable for physiopharmacological studies dealing with regulation of voiding cycle.
Authors:
C A Maggi; P Santicioli; A Meli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pharmacological methods     Volume:  15     ISSN:  0160-5402     ISO Abbreviation:  J Pharmacol Methods     Publication Date:  1986 Apr 
Date Detail:
Created Date:  1986-05-23     Completed Date:  1986-05-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7806596     Medline TA:  J Pharmacol Methods     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  157-67     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anesthesia
Animals
Cystoscopes
Female
Hexamethonium Compounds / pharmacology
Male
Muscle Contraction / drug effects
Muscle, Smooth / physiology
Pressure
Rats
Rats, Inbred Strains
Sex Factors
Urethane
Urinary Bladder / physiology*
Chemical
Reg. No./Substance:
0/Hexamethonium Compounds; 51-79-6/Urethane

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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