Document Detail

The nonrandom distribution of long clusters of all possible classes of trinucleotide repeats in barley chromosomes.
MedLine Citation:
PMID:  17874212     Owner:  NLM     Status:  MEDLINE    
This paper is the first to report the long-range organization of all possible classes of trinucleotide motifs in a higher plant genome. Fluorescent in situ hybridization (FISH), employing the synthetic oligonucleotides (AAC)5, (AAG)5, (AAT)5, (AGG)5, (CAC)5, (CAT)5, (CAG)5, (ACT)5, (ACG)5 and (GCC)5, was used to characterize the nonrandom and motif-dependent distribution of tandem arrays of trinucleotide repeats in the metaphase chromosomes and interphase nuclei of barley (Hordeum vulgare L.). This provided detailed information on the sequence content of barley chromatin and allowed the saturation of the physical map of all barley chromosomes. The following conclusions were also drawn: (1) Except for (AAT)5 and (GCC)5, the studied repetitive motifs have a characteristic pattern of distribution in terms of their in situ FISH signals. Some permit the accurate identification of individual chromosomes. (2) (CAG)5, (CAT)5 and (ACT)5 are not found in all barley chromosomes. (3) With the exception of (ACT)5, the remaining trinucleotide repeats occur predominantly in the heterochromatin and are largely absent from the euchromatic regions. Moreover, (CAC)5, (ACG)5 and (CAG)5 are exclusively concentrated in the centromeres. The employment of simple synthetic probes for the identification of chromosomes and genomic characterization, and their importance in studies on genome organization, function and evolution, are discussed.
Angeles Cuadrado; Nicolas Jouve
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-23
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  15     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  2007  
Date Detail:
Created Date:  2007-09-17     Completed Date:  2007-12-14     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  711-20     Citation Subset:  IM    
Department of Cell Biology and Genetics, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain.
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MeSH Terms
Amino Acid Motifs
Chromosomes, Plant / ultrastructure*
Cluster Analysis
Euchromatin / genetics
Evolution, Molecular
Genes, Plant
Genome, Plant
Heterochromatin / genetics
Hordeum / genetics*
In Situ Hybridization, Fluorescence
Models, Genetic
Physical Chromosome Mapping
Trinucleotide Repeat Expansion*
Trinucleotide Repeats / genetics*
Reg. No./Substance:
0/Euchromatin; 0/Heterochromatin

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