| The nonrandom distribution of long clusters of all possible classes of trinucleotide repeats in barley chromosomes. | |
MedLine Citation:
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PMID: 17874212 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This paper is the first to report the long-range organization of all possible classes of trinucleotide motifs in a higher plant genome. Fluorescent in situ hybridization (FISH), employing the synthetic oligonucleotides (AAC)5, (AAG)5, (AAT)5, (AGG)5, (CAC)5, (CAT)5, (CAG)5, (ACT)5, (ACG)5 and (GCC)5, was used to characterize the nonrandom and motif-dependent distribution of tandem arrays of trinucleotide repeats in the metaphase chromosomes and interphase nuclei of barley (Hordeum vulgare L.). This provided detailed information on the sequence content of barley chromatin and allowed the saturation of the physical map of all barley chromosomes. The following conclusions were also drawn: (1) Except for (AAT)5 and (GCC)5, the studied repetitive motifs have a characteristic pattern of distribution in terms of their in situ FISH signals. Some permit the accurate identification of individual chromosomes. (2) (CAG)5, (CAT)5 and (ACT)5 are not found in all barley chromosomes. (3) With the exception of (ACT)5, the remaining trinucleotide repeats occur predominantly in the heterochromatin and are largely absent from the euchromatic regions. Moreover, (CAC)5, (ACG)5 and (CAG)5 are exclusively concentrated in the centromeres. The employment of simple synthetic probes for the identification of chromosomes and genomic characterization, and their importance in studies on genome organization, function and evolution, are discussed. |
Authors:
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Angeles Cuadrado; Nicolas Jouve |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-08-23 |
Journal Detail:
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Title: Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Volume: 15 ISSN: 0967-3849 ISO Abbreviation: Chromosome Res. Publication Date: 2007 |
Date Detail:
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Created Date: 2007-09-17 Completed Date: 2007-12-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9313452 Medline TA: Chromosome Res Country: Netherlands |
Other Details:
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Languages: eng Pagination: 711-20 Citation Subset: IM |
Affiliation:
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Department of Cell Biology and Genetics, University of Alcalá, 28871 Alcalá de Henares, Madrid, Spain. angeles.cuadrado@uah.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Motifs Chromosomes, Plant / ultrastructure* Cluster Analysis Euchromatin / genetics Evolution, Molecular Genes, Plant Genome, Plant Genomics Heterochromatin / genetics Hordeum / genetics* In Situ Hybridization, Fluorescence Models, Genetic Physical Chromosome Mapping Trinucleotide Repeat Expansion* Trinucleotide Repeats / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Euchromatin; 0/Heterochromatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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