Document Detail


A non-toxic fluorogenic dye for mitochondria labeling.
MedLine Citation:
PMID:  23850639     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Mitochondria, powerhouses of cells, are responsible for many critical cellular functions, such as cell energy metabolism, reactive oxygen species production, and apoptosis regulation. Monitoring mitochondria morphology in live cells temporally and spatially could help with the understanding of the mechanisms of mitochondrial functional regulation and the pathogenesis of mitochondria-related diseases.
METHODS: A novel non-cytotoxic fluorogenic compound, AcQCy7, was developed as a mitochondria-specific dye.
RESULTS: AcQCy7 emitted no fluorescent signal outside of cells, but it became fluorescent after intracellular hydrolysis of the acetyl group. The hydrolyzed fluorescent product was well retained in mitochondria, enabling long-lasting fluorescence imaging of mitochondria without cell washing. A 2-day culture study using AcQCy7 showed no sign of cytotoxicity, whereas a commonly used mitochondria-staining probe, Mitochondria Tracker Green, caused significant cell death even at a much lower concentration. Apoptosis-causing mitochondria fission was monitored clearly in real time by AcQCy7.
CONCLUSIONS: A simple add-and-read mitochondria specific dye AcQCy7 has been validated in various cell models. Bright mitochondria specific fluorescent signal in treated cells lasted several days without noticeable toxicity.
GENERAL SIGNIFICANCE: The probe AcQCy7 has been proofed to be a non-toxic agent for long-term mitochondria imaging.
Authors:
Junyan Han; Myung Shin Han; Ching-Hsuan Tung
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-07-12
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1830     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-09-24     Completed Date:  2014-03-21     Revised Date:  2014-11-04    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  5130-5     Citation Subset:  IM    
Copyright Information:
© 2013.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / physiology
Cell Death / physiology
Cell Line
Cell Line, Tumor
Fluorescent Dyes / chemistry*,  metabolism
HeLa Cells
Humans
Mitochondria / chemistry*,  metabolism
Optical Imaging / methods*
Staining and Labeling / methods
Grant Support
ID/Acronym/Agency:
CA135312/CA/NCI NIH HHS; P30 CA016672/CA/NCI NIH HHS; R01 CA135312/CA/NCI NIH HHS; R01 GM094880/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fluorescent Dyes
Comments/Corrections

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