Document Detail


A non-redundant role for OX40 in the competitive fitness of Treg in response to IL-2.
MedLine Citation:
PMID:  20806292     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OX40 stimulation is known to enhance activation of effector T cells and to inhibit induction and suppressive function of Treg. Here we uncovered a novel role of OX40 in sustaining Treg competitive fitness in vivo, during repopulation of lymphopenic hosts and reconstitution of BM chimeras. Defective expansion of OX40-null Treg diminished their ability to suppress inflammation in a model of lymphopenia-driven colitis. OX40-mediated promotion of Treg fitness spanned beyond lymphopenic environments, as endogenous Treg in OX40-null mice showed decreased accumulation during thymic development, enhanced susceptibility to antibody-mediated depletion and defective turnover following thymectomy. In vitro, OX40-deficient Treg were found to be intrinsically hyporesponsive to IL-2, in terms of Stat5 phosphorylation and proliferation, according to elevated SOCS1 content and reduced miR155 expression. Therefore, OX40 is a key factor in shaping Treg sensitivity to IL-2 and promoting their proliferation and survival, toward accurate immune regulation.
Authors:
Silvia Piconese; Paola Pittoni; Alessia Burocchi; Andrea Gorzanelli; Alessandra Carè; Claudio Tripodo; Mario P Colombo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  40     ISSN:  1521-4141     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-11-01     Completed Date:  2010-12-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  2902-13     Citation Subset:  IM    
Affiliation:
Molecular Immunology Unit, Department of Experimental Oncology, Fondazione IRCCS Istituto Nazionale dei Tumori, Milan, Italy.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Cell Proliferation
Colitis / immunology*
Flow Cytometry
Interleukin-2 / immunology*
Lymphopenia / immunology*
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Phosphorylation / immunology
Radiation Chimera
Receptors, OX40 / immunology*
STAT5 Transcription Factor / immunology
Specific Pathogen-Free Organisms
Suppressor of Cytokine Signaling Proteins / immunology
T-Lymphocytes, Regulatory / immunology*
Chemical
Reg. No./Substance:
0/Interleukin-2; 0/Receptors, OX40; 0/STAT5 Transcription Factor; 0/Socs1 protein, mouse; 0/Suppressor of Cytokine Signaling Proteins; 0/Tnfrsf4 protein, mouse

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