Document Detail

The non-psychoactive cannabidiol triggers caspase activation and oxidative stress in human glioma cells.
MedLine Citation:
PMID:  16909207     Owner:  NLM     Status:  MEDLINE    
Recently, we have shown that the non-psychoactive cannabinoid compound cannabidiol (CBD) induces apoptosis of glioma cells in vitro and tumor regression in vivo. The present study investigated a possible involvement of caspase activation and reactive oxygen species (ROS) induction in the apoptotic effect of CBD. CBD produced a gradual, time-dependent activation of caspase-3, which preceded the appearance of apoptotic death. In addiction, release of cytochrome c and caspase-9 and caspase-8 activation were detected. The exposure to CBD caused in glioma cells an early production of ROS, depletion of intracellular glutathione and increase activity of glutathione reductase and glutathione peroxidase enzymes. Under the same experimental condition, CBD did not impair primary glia. Thus, we found a different sensitivity to the anti-proliferative effect of CBD in human glioma cells and non-transformed cells that appears closely related to a selective ability of CBD in inducing ROS production and caspase activation in tumor cells.
P Massi; A Vaccani; S Bianchessi; B Costa; P Macchi; D Parolaro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular and molecular life sciences : CMLS     Volume:  63     ISSN:  1420-682X     ISO Abbreviation:  Cell. Mol. Life Sci.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-08     Completed Date:  2006-10-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9705402     Medline TA:  Cell Mol Life Sci     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  2057-66     Citation Subset:  IM    
Department of Pharmacology, Chemotherapy and Medical Toxicology, University of Milan, via Vanvitelli 32, 20129 Milan, Italy.
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MeSH Terms
Cannabidiol / pharmacology*
Caspases / metabolism*
Cell Line, Tumor
Cells, Cultured
Enzyme Activation
Glioma / metabolism*
Glutathione / metabolism
Oxidative Stress*
Reactive Oxygen Species / metabolism*
Reg. No./Substance:
0/Reactive Oxygen Species; 13956-29-1/Cannabidiol; 70-18-8/Glutathione; EC 3.4.22.-/Caspases

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