Document Detail


A non-invasive test for assessing embryo potential by gene expression profiles of human cumulus cells: a proof of concept study.
MedLine Citation:
PMID:  19028806     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Identification of new criteria for embryo quality is required to improve the clinical outcome of in vitro fertilization. The aim of this study was to determine the gene expression profile of cumulus cells (CC) surrounding the oocyte as biomarkers for embryo potential and to identify genes to be used as prognostic indicators of successful pregnancy. CC from single oocytes were analysed using DNA microarrays. Gene expression profiles of CC surrounding the oocyte associated with good embryonic quality and pregnancy outcome were computed. We observed that CC issued from oocytes that developed into embryos with a good morphology had differing gene expression profile according to the pregnancy outcome of the embryo. We demonstrated that the expression of BCL2L11, PCK1 and NFIB in CC is significantly correlated with embryo potential and successful pregnancy. These results were confirmed by quantitative RT-PCR. The gene expression profiling of human CC correlates with embryo potential and pregnancy outcome. BCL2L11, PCK1 and NFIB genes are proposed as biomarkers for predicting pregnancy. Our findings suggest a non-invasive approach, offering a new potential strategy for competent embryo selection. This approach should be validated in single-embryo transfer programmes.
Authors:
S Assou; D Haouzi; K Mahmoud; A Aouacheria; Y Guillemin; V Pantesco; T Rème; H Dechaud; J De Vos; S Hamamah
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Publication Detail:
Type:  Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-21
Journal Detail:
Title:  Molecular human reproduction     Volume:  14     ISSN:  1460-2407     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-01-08     Completed Date:  2009-02-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  711-9     Citation Subset:  IM    
Affiliation:
CHU Montpellier, Institut de Recherche en Biothérapie, Hôpital Saint-Eloi, Montpellier F-34000, France.
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MeSH Terms
Descriptor/Qualifier:
Adult
Biological Markers / metabolism*
Cumulus Cells / physiology*
Embryo, Mammalian / physiology*
Female
Fertilization in Vitro
Gene Expression Profiling*
Gene Regulatory Networks
Humans
Oligonucleotide Array Sequence Analysis*
Pregnancy
Pregnancy Outcome / genetics
Retrospective Studies
Chemical
Reg. No./Substance:
0/Biological Markers
Comments/Corrections
Comment In:
Mol Hum Reprod. 2008 Dec;14(12):665   [PMID:  19022869 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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