| A non-canonical UBA-UBL interaction forms the linear-ubiquitin-chain assembly complex. | |
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MedLine Citation:
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PMID: 22430200 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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HOIL-1L and its binding partner HOIP are essential components of the E3-ligase complex that generates linear ubiquitin (Ub) chains, which are critical regulators of NF-κB activation. Using crystallographic and mutational approaches, we characterize the unexpected structural basis for the specific interaction between the Ub-like domain (UBL) of HOIL-1L and the Ub-associated domain (UBA) of HOIP. Our data indicate the functional significance of this non-canonical mode of UBA-UBL interaction in E3 complex formation and subsequent NF-κB activation. This study highlights the versatility and specificity of protein-protein interactions involving Ub/UBLs and their cognate proteins. |
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Authors:
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Hirokazu Yagi; Kazuhiro Ishimoto; Takeshi Hiromoto; Hiroaki Fujita; Tsunehiro Mizushima; Yoshinori Uekusa; Maho Yagi-Utsumi; Eiji Kurimoto; Masanori Noda; Susumu Uchiyama; Fuminori Tokunaga; Kazuhiro Iwai; Koichi Kato |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2012-05-01 |
Journal Detail:
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Title: EMBO reports Volume: 13 ISSN: 1469-3178 ISO Abbreviation: EMBO Rep. Publication Date: 2012 May |
Date Detail:
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Created Date: 2012-05-01 Completed Date: 2012-08-29 Revised Date: 2013-05-02 |
Medline Journal Info:
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Nlm Unique ID: 100963049 Medline TA: EMBO Rep Country: England |
Other Details:
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Languages: eng Pagination: 462-8 Citation Subset: IM |
Affiliation:
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Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carrier Proteins
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chemistry,
metabolism Cell Line Circular Dichroism Humans Immunoprecipitation Magnetic Resonance Spectroscopy NF-kappa B / metabolism Protein Binding Protein Structure, Secondary Surface Plasmon Resonance Ubiquitin / chemistry*, metabolism* Ubiquitin-Protein Ligases / chemistry*, metabolism* Ultracentrifugation |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/NF-kappa B; 0/RNF31 protein, human; 0/Ubiquitin; EC 6.3.2.19/HOIL-1 protein, human; EC 6.3.2.19/Ubiquitin-Protein Ligases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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