Document Detail

A non-alphoid repetitive DNA sequence from human chromosome 21.
MedLine Citation:
PMID:  1634230     Owner:  NLM     Status:  MEDLINE    
A non-alphoid repetitive DNA from human chromosome 22, consisting of a 48-bp motif, shows homology to both G-group chromosomes in the gorilla, thus indicating the presence of additional repeat family members on further human chromosomes. Therefore, we screened a chromosome-21-specific cosmid library using this repetitive sequence from chromosome 22 (D22Z3). Some 40-50 cosmid clones were positive in tests for hybridization. One of the clones giving the strongest signals was digested with EcoRI/PstI, which we knew to cut frequently within the repeats; this resulted in fragments containing repeat units only. The fragments were subcloned into plasmid vector pTZ 19. Sequence-analysis of a 500-bp insert showed ten copies of a 48-bp repeat similar to D22Z3, with about 15% sequence deviation from the chromosome 22 consensus sequence. In situ hybridization of the newly isolated recombinant established its chromosome 21 specificity at high stringency. Physical mapping by pulsed field gel electrophoresis placed this new repeat in close vicinity to the chromosome 21 alphoid repeat. No cross-hybridization with other mammalian genomes except for those of apes was observed. The locus has been designated D21Z2 by the Genome Data Base. A gel mobility shift assay indicated that this repetitive motif has protein-binding properties.
R Müllenbach; S Lutz; K Holzmann; S Dooley; N Blin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Human genetics     Volume:  89     ISSN:  0340-6717     ISO Abbreviation:  Hum. Genet.     Publication Date:  1992 Jul 
Date Detail:
Created Date:  1992-08-25     Completed Date:  1992-08-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7613873     Medline TA:  Hum Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  519-23     Citation Subset:  IM    
Institut für Humangenetik, Universität des Saarlandes, Homburg/Saar, Federal Republic of Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M75879;  M76594;  S78409;  S78411;  S78413;  S78753;  S83243;  S89848;  S89851;  S89853;  S89910
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MeSH Terms
Base Sequence
Centromere / chemistry
Chromosome Aberrations*
Chromosomes, Human, Pair 21*
Cloning, Molecular
DNA / analysis*
DNA Probes*
Electrophoresis, Gel, Pulsed-Field
Molecular Sequence Data
Nucleic Acid Hybridization
Repetitive Sequences, Nucleic Acid / genetics*
Restriction Mapping
Sequence Homology, Nucleic Acid
Species Specificity
Reg. No./Substance:
0/DNA Probes; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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