Document Detail


nm23-H1 suppresses invasion of oral squamous cell carcinoma-derived cell lines without modifying matrix metalloproteinase-2 and matrix metalloproteinase-9 expression.
MedLine Citation:
PMID:  11337376     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
nm23-H1 is a candidate gene for the suppression of cancer metastasis. Several studies on human breast, hepatocellular, gastric, ovarian, and colon carcinomas and melanomas have shown that reduced nm23-H1 expression was closely related to metastatic progression with poor prognosis. However, the biochemical mechanism by which nm23-H1 suppresses the metastasis has yet to be elucidated. In this study, we analyzed the correlation between nm23 expression, cell motility, and the invasive abilities of six different oral squamous cell carcinoma cell lines (HSC2, HSC3, HSC4, KB, OSC19, and OSC20). Reduced mRNA/protein expression of the nm23-H1 was observed in three cell lines (HSC2, HSC3, and HSC4). These cell lines exhibited increased cell motility and an invasive character on organotypic raft culture. On the other hand, the cell lines (KB, OSC19, and OSC20) that showed a higher expression of nm23-H1 exhibited a threefold to fivefold reduced motility and also reflected fewer invasions compared to the former three cell lines. Because the HSC3 cells demonstrated the lowest nm23-H1 expression with the highest cell motility and invasive character, we established nm23-H1-transfected HSC3 cell lines to investigate whether exogenous nm23-H1 protein could inhibit cell migration and invasive activity. These transfectants showed a significant reduction in cell motility with exogenous nm23-H1 in a dose-dependent manner, and exhibited a noninvasive character. An immunofluorescence study demonstrated a distinct stress-fiber distribution at peripheral region of these transfectants. However, no significant difference of matrix metalloproteinase (MMP)-2 and MMP-9 expression was observed between mock transfectant and nm23-H1-transfected cells. These findings suggest that nm23-H1 inhibits the invasive activity of oral squamous cell carcinoma by suppression of cell motility without altering the MMP-2 and MMP-9 status.
Authors:
M H Khan; M Yasuda; F Higashino; S Haque; T Kohgo; M Nakamura; M Shindoh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American journal of pathology     Volume:  158     ISSN:  0002-9440     ISO Abbreviation:  Am. J. Pathol.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-04     Completed Date:  2001-06-21     Revised Date:  2013-06-11    
Medline Journal Info:
Nlm Unique ID:  0370502     Medline TA:  Am J Pathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1785-91     Citation Subset:  AIM; IM    
Affiliation:
Department of Oral Pathobiological Science, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Squamous Cell / enzymology,  pathology*
Cell Movement
Gelatin / metabolism
Gene Expression Regulation, Neoplastic
Humans
Matrix Metalloproteinase 2 / metabolism*
Matrix Metalloproteinase 9 / metabolism*
Microscopy, Confocal
Monomeric GTP-Binding Proteins / genetics,  physiology*
Mouth Neoplasms / enzymology,  pathology*
NM23 Nucleoside Diphosphate Kinases
Nucleoside-Diphosphate Kinase*
Transcription Factors / genetics,  physiology*
Transfection
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/NM23 Nucleoside Diphosphate Kinases; 0/Transcription Factors; 9000-70-8/Gelatin; EC 2.7.4.6/NME1 protein, human; EC 2.7.4.6/Nucleoside-Diphosphate Kinase; EC 3.4.24.24/Matrix Metalloproteinase 2; EC 3.4.24.35/Matrix Metalloproteinase 9; EC 3.6.5.2/Monomeric GTP-Binding Proteins
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