Document Detail

The nitrate reductase-encoding gene of Volvox carteri: map location, sequence and induction kinetics.
MedLine Citation:
PMID:  1398126     Owner:  NLM     Status:  MEDLINE    
The nitrate reductase (NR) structural gene (nitA) of Volvox carteri has been cloned and characterized. There is a single copy of this gene in the genome, and RFLP (restriction-fragment length polymorphism) analysis assigns it to the previously defined nitA/chlR locus on linkage group IX, 20-30 cM from the two beta-tubulin-encoding loci. Determination of the 5871-nt sequence of the coding region of genomic clones, and comparisons to a cDNA sequence, revealed ten introns and eleven exons that encode a 864-aa polypeptide. Detailed comparisons with higher-plant and fungal NRs indicate that, whereas the aa sequence is strongly conserved within functional domains for the flavin adenine dinucleotide-, heme- and molybdenum-pterin cofactor-binding sites, substantial differences in the aa sequence occur in the N-terminal end and the two inter-domain regions. Two potential transcription start points 439 and 452 nt upstream from the start codon and a polyadenylation signal 355 nt downstream from the stop codon have been identified by primer-extension analysis and cDNA sequencing, respectively. Accumulation of the nitA transcript is both induced by nitrate and repressed by ammonium and urea: after the organism is transferred from ammonium to nitrate as the nitrogen source, a 3.6-kb NR transcript is readily detectable on Northern blots by 10 min, reaches maximum abundance by 30 min, and then rapidly declines to an intermediate level that is subsequently maintained. Substantial induction by nitrate is observed at the end of the dark portion of the daily light/dark cycle, but the inductive response peaks in the first hour of the light period.(ABSTRACT TRUNCATED AT 250 WORDS)
H Gruber; S D Goetinck; D L Kirk; R Schmitt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Gene     Volume:  120     ISSN:  0378-1119     ISO Abbreviation:  Gene     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-11-04     Completed Date:  1992-11-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7706761     Medline TA:  Gene     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  75-83     Citation Subset:  IM    
Lehrstuhl für Genetik, Universität Regensburg, Germany.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/M79310;  X64136;  X65053;  X65107;  X65108;  X65109;  X65110;  X65111;  X65112;  X65113
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MeSH Terms
Algae, Green / enzymology*,  genetics*
Amino Acid Sequence
Base Sequence
Chromosome Mapping
Enzyme Induction / genetics
Molecular Sequence Data
Nitrate Reductase
Nitrate Reductases / biosynthesis*,  chemistry,  genetics*
Sequence Homology, Amino Acid
Grant Support
Reg. No./Substance:
EC 1.7.-/Nitrate Reductases; EC Reductase

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