Document Detail


A newly identified bacterial cell-penetrating peptide that reduces the transcription of pro-inflammatory cytokines.
MedLine Citation:
PMID:  20554895     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell-permeable proteins, also called cell-penetrating peptides (CPPs), have the ability to cross cellular membranes, either alone or in association with bioactive cargo. We identified the Yersinia protein YopM as a novel bacterial cell-permeable protein. Here, we describe the ability of isolated recombinant YopM to enter host cells without a requirement for additional factors. This autonomous translocation of YopM was confirmed in several cell types, indicating that it is an intrinsic property of YopM. Using truncated versions of YopM, we show that either of the two N-terminal alpha-helices of YopM mediates translocation into the cells. Furthermore, the two alpha-helices are also able to deliver heterologous cargo, such as GFP or YopE. In addition, we found that, after entering the cells, YopM is functional and efficiently downregulates the transcription of pro-inflammatory cytokines (such as tumor necrosis factor-alpha and interleukins 12, 15 and 18). This finding suggests the potential use of YopM as a tool for protein delivery. Furthermore, it can lead to important advances in understanding and evaluating the intracellular and molecular function of YopM without the need for infection with Yersinia.
Authors:
Christian Rüter; Christoph Buss; Julia Scharnert; Gerhard Heusipp; M Alexander Schmidt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  123     ISSN:  1477-9137     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-17     Completed Date:  2010-10-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  2190-8     Citation Subset:  IM    
Affiliation:
Westfälische Wilhelms-Universität Münster, Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung (ZMBE), Von-Esmarch-Strasse 56, D-48149 Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Outer Membrane Proteins / chemistry,  genetics,  metabolism*
Cytokines / genetics,  immunology*
Endoplasmic Reticulum / metabolism
Green Fluorescent Proteins / metabolism
HL-60 Cells
Hela Cells
Humans
Inflammation / immunology*
Membrane Microdomains / metabolism
Protein Structure, Secondary
Recombinant Fusion Proteins / genetics,  metabolism
Transcription, Genetic*
Tumor Necrosis Factor-alpha / genetics,  metabolism
Yersinia enterocolitica / genetics,  metabolism*,  pathogenicity
Yersinia pestis / metabolism,  pathogenicity
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Cytokines; 0/Recombinant Fusion Proteins; 0/Tumor Necrosis Factor-alpha; 124893-29-4/yopM protein, Yersinia; 147336-22-9/Green Fluorescent Proteins

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