| A newly identified bacterial cell-penetrating peptide that reduces the transcription of pro-inflammatory cytokines. | |
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MedLine Citation:
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PMID: 20554895 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell-permeable proteins, also called cell-penetrating peptides (CPPs), have the ability to cross cellular membranes, either alone or in association with bioactive cargo. We identified the Yersinia protein YopM as a novel bacterial cell-permeable protein. Here, we describe the ability of isolated recombinant YopM to enter host cells without a requirement for additional factors. This autonomous translocation of YopM was confirmed in several cell types, indicating that it is an intrinsic property of YopM. Using truncated versions of YopM, we show that either of the two N-terminal alpha-helices of YopM mediates translocation into the cells. Furthermore, the two alpha-helices are also able to deliver heterologous cargo, such as GFP or YopE. In addition, we found that, after entering the cells, YopM is functional and efficiently downregulates the transcription of pro-inflammatory cytokines (such as tumor necrosis factor-alpha and interleukins 12, 15 and 18). This finding suggests the potential use of YopM as a tool for protein delivery. Furthermore, it can lead to important advances in understanding and evaluating the intracellular and molecular function of YopM without the need for infection with Yersinia. |
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Authors:
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Christian Rüter; Christoph Buss; Julia Scharnert; Gerhard Heusipp; M Alexander Schmidt |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of cell science Volume: 123 ISSN: 1477-9137 ISO Abbreviation: J. Cell. Sci. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-17 Completed Date: 2010-10-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0052457 Medline TA: J Cell Sci Country: England |
Other Details:
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Languages: eng Pagination: 2190-8 Citation Subset: IM |
Affiliation:
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Westfälische Wilhelms-Universität Münster, Institut für Infektiologie, Zentrum für Molekularbiologie der Entzündung (ZMBE), Von-Esmarch-Strasse 56, D-48149 Münster, Germany. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Outer Membrane Proteins
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chemistry,
genetics,
metabolism* Cytokines / genetics, immunology* Endoplasmic Reticulum / metabolism Green Fluorescent Proteins / metabolism HL-60 Cells Hela Cells Humans Inflammation / immunology* Membrane Microdomains / metabolism Protein Structure, Secondary Recombinant Fusion Proteins / genetics, metabolism Transcription, Genetic* Tumor Necrosis Factor-alpha / genetics, metabolism Yersinia enterocolitica / genetics, metabolism*, pathogenicity Yersinia pestis / metabolism, pathogenicity |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Outer Membrane Proteins; 0/Cytokines; 0/Recombinant Fusion Proteins; 0/Tumor Necrosis Factor-alpha; 124893-29-4/yopM protein, Yersinia; 147336-22-9/Green Fluorescent Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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