Document Detail


A new yeast endosomal SNARE related to mammalian syntaxin 8.
MedLine Citation:
PMID:  12453154     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report the identification of a yeast SNARE that has escaped notice because of an apparent error in the genome sequence and because it is functionally redundant. It is encoded by an extended version of ORF YAL014c, and since its SNARE motif is related to mammalian syntaxin 8 we term the gene SYN8. Syn8p is in endosomes. Co-precipitation indicates a set of complexes containing Pep12p, Vti1p, either Syn8p or Tlg1p and either Snc1p or Ykt6p. Analysis of growth and trafficking defects demonstrates that in the absence of Tlg1p, Syn8p is required for Pep12p function. Conversely, when Tlg1p is present, Syn8p can be removed without loss of Pep12p function, or induction of any other obvious trafficking defect. Syn8p thus appears to be a functional homolog of mammalian syntaxin 8, but Tlg1p can, amongst other roles, provide an equivalent function.
Authors:
Michael J Lewis; Hugh R B Pelham
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Traffic (Copenhagen, Denmark)     Volume:  3     ISSN:  1398-9219     ISO Abbreviation:  Traffic     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-27     Completed Date:  2003-06-16     Revised Date:  2006-05-01    
Medline Journal Info:
Nlm Unique ID:  100939340     Medline TA:  Traffic     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  922-9     Citation Subset:  IM    
Affiliation:
MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Motifs
Amino Acid Sequence
Animals
Endosomes / metabolism
Green Fluorescent Proteins
Immunoblotting
Luminescent Proteins / metabolism
Membrane Proteins / chemistry*,  metabolism*,  physiology*
Microscopy, Confocal
Microscopy, Fluorescence
Molecular Sequence Data
Plasmids / metabolism
Protein Binding
Qa-SNARE Proteins
R-SNARE Proteins
Recombinant Fusion Proteins / metabolism
SNARE Proteins
Saccharomyces cerevisiae Proteins / metabolism
Schizosaccharomyces
Vesicular Transport Proteins*
Chemical
Reg. No./Substance:
0/Luminescent Proteins; 0/Membrane Proteins; 0/PEP12 protein, S cerevisiae; 0/Qa-SNARE Proteins; 0/R-SNARE Proteins; 0/Recombinant Fusion Proteins; 0/SNARE Proteins; 0/SNC1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Vesicular Transport Proteins; 0/YKT6 protein, S cerevisiae; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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