| A new terbium(III) chelate as an efficient singlet oxygen fluorescence probe. | |
| | |
MedLine Citation:
|
PMID: 16632124 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A terbium(III) chelate fluorescence probe for detection of singlet oxygen (1O2) in aqueous media, N,N,N1,N1-[2,6-bis(3'-aminomethyl-1'-pyrazolyl)-4-(9''-anthryl)pyridine] tetrakis (acetate)-Tb3+ (PATA-Tb3+), was designed and synthesized. The new chelate is highly water soluble, is almost nonfluorescent, and can specifically react with 1O2 to yield a strongly fluorescent chelate, the endoperoxide of PATA-Tb3+, accompanied by a remarkable increase in the fluorescence quantum yield from 0.46 to 10.5%. The long fluorescence lifetime of the endoperoxide (2.76 ms) allows the probe to be used favorably for time-resolved fluorescence detection of 1O2. The studies of fluorescence property and reaction specificity indicate that the new probe is highly sensitive and selective for 1O2. The probe was used for quantitative detection of 1O2 generated from a MoO4(2-) -H2O2 system to give a detection limit of 10.8 nM. In addition, the good applicability of the probe was demonstrated by the real-time monitoring of the kinetic process of 1O2 generation in a horseradish peroxidase-catalyzed oxidation system of indole-3-acetic acid in a weakly acidic buffer. |
| | |
Authors:
|
Mingqian Tan; Bo Song; Guilan Wang; Jingli Yuan |
Related Documents
:
|
1277124 - Interaction of the carcinogen 3,3-dimethyl-1-phenyltriazene with nucleic acids of vario... 11475694 - Purification of nucleic acids by hybridization to affinity tagged pna probes. 6164404 - The reaction of thymocytes to hypoxic stress. 17970584 - Determination of the optimized conditions for coupling oligonucleotides with 16-mercapt... 16263304 - Interactions of tannic acid and its derivatives (ellagic and gallic acid) with calf thy... 17513434 - Lysine, arginine, and related amino acids: an introduction to the 6th amino acid assess... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-20 |
Journal Detail:
|
Title: Free radical biology & medicine Volume: 40 ISSN: 0891-5849 ISO Abbreviation: Free Radic. Biol. Med. Publication Date: 2006 May |
Date Detail:
|
Created Date: 2006-04-24 Completed Date: 2006-08-29 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8709159 Medline TA: Free Radic Biol Med Country: United States |
Other Details:
|
Languages: eng Pagination: 1644-53 Citation Subset: IM |
Affiliation:
|
Department of Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Sciences, Dalian 116023, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Fluorescent Dyes
/
chemical synthesis* Indoleacetic Acids / metabolism Organometallic Compounds / chemical synthesis* Sensitivity and Specificity Singlet Oxygen / analysis* Terbium* |
| Chemical | |
Reg. No./Substance:
|
0/Fluorescent Dyes; 0/Indoleacetic Acids; 0/N,N,N1,N1-(2,6-bis(3'-aminomethyl-1'-pyrazolyl)-4-(9''-anthryl)pyridine)tetrakis (acetate)-terbium(III); 0/Organometallic Compounds; 17778-80-2/Singlet Oxygen; 7440-27-9/Terbium; 87-51-4/indoleacetic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Concentrations of iron correlate with the extent of protein, but not lipid, oxidation in advanced hu...
Next Document: Metalloprotein-dependent decomposition of S-nitrosothiols: studies on the stabilization and measurem...