Document Detail


A new technique of coronary artery ligation: experimental myocardial infarction in rats in vivo with reduced mortality.
MedLine Citation:
PMID:  9406166     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In vivo models of myocardial infarction following coronary artery ligation in the rat still suffer from high early mortality and a low rate of success of myocardial infarction. This study investigated the possibility of reducing early mortality and increasing the rate of myocardial infarction by modifications of surgical techniques. Eighteen rats were divided into two groups: normal control (3 rats) and ligation (15 rats). The major modifications of surgical techniques used in this study include: (1) no exteriorization of the heart, (2) ligation of the origins of the branches rather than the main trunk of the left coronary artery, (3) removal of air from the chest after closure, (4) supplying oxygen immediately after extubation. Following surgery, the rats recovered uneventfully and 11 rats were alive after 16 weeks. One rat, with a large myocardial infarction, died 2 h after surgery. Early mortality (during surgery and 1 week after surgery) was 6.7% with a success rate of myocardial infarction of 85%. The left ventricle in the ligation group showed significant dilation relative to normal and sham-operated control hearts (317% of control hearts, p < 0.001). However, myocardial mass did not increase. The average infarct size was 33%. These results demonstrate that a reduction in early mortality and an increased success rate of myocardial infarction can be achieved by modifications of surgical techniques.
Authors:
J Ye; L Yang; R Sethi; J Copps; B Ramjiawan; R Summers; R Deslauriers
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  176     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-01-23     Completed Date:  1998-01-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  227-33     Citation Subset:  IM    
Affiliation:
Institute for Biodiagnostics, National Research Council Canada, Winnipeg, Manitoba, Canada.
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Cardiac Surgical Procedures / methods*
Coronary Circulation
Coronary Disease / pathology,  physiopathology*
Female
Heart Ventricles / pathology,  physiopathology
Ligation / methods,  mortality
Male
Myocardial Infarction / pathology,  physiopathology*
Rats
Rats, Sprague-Dawley
Survival Rate
Ventricular Function, Left / physiology

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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