| A new system for the study of erythroid cell differentiation. | |
| | |
MedLine Citation:
|
PMID: 6321217 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Highly purified erythroid progenitor cells (CFU-E), were cultured in vitro for 48 h. Cell division was monitored regularly and cells were isolated at times when maximal colony formation occurred. The cell mixture after one and two cell divisions consisted mainly of proerythroblasts and basophilic erythroblasts (early erythroid cells). After four cell divisions, predominantly polychromatic and orthochromatic cells (late erythroid) were present. After two days of culture, the majority of inoculated cells had differentiated into reticulocytes. This time course in vitro was comparable to the differentiation of CFU-E in vivo. As judged by cytochrome-c oxidase activity, there was an increase in total mitochondrial activity per culture; however, the activity per cell decreased during the differentiation process. The formation of hemoglobin started after the first cell division. This system is presented as a new tool for the study of biochemical processes accompanying erythroid cell differentiation. |
| | |
Authors:
|
W Nijhof; P K Wierenga |
Related Documents
:
|
18202877 - Evolution of cell division in bacteria. 12228357 - Stunted plant 1, a gene required for expansion in rapidly elongating but not in dividin... 1368817 - Determination of division rates of rcho cells in high density and immobilized fermentat... 18511557 - Asymmetric mitosis: unequal segregation of proteins destined for degradation. 12442907 - A glucose-inducible gene in schizosaccharomyces pombe, rrg1+, is involved in negative r... 8071377 - In vitro study of the intrinsic toxicity of synthetic surfaces to cells. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Experimental hematology Volume: 12 ISSN: 0301-472X ISO Abbreviation: Exp. Hematol. Publication Date: 1984 Feb |
Date Detail:
|
Created Date: 1984-04-20 Completed Date: 1984-04-20 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0402313 Medline TA: Exp Hematol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 115-20 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cattle Cell Differentiation Colony-Forming Units Assay Electron Transport Complex IV / blood Erythrocytes / cytology*, enzymology Hemoglobins / biosynthesis Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Hemoglobins; EC 1.9.3.1/Electron Transport Complex IV |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Estimation of hemopoietic potential by CFU-c and bone marrow scan in cancer patients.
Next Document: Bronchopulmonary obstruction in children with respiratory virus infections.