Document Detail

A new synthetic RNA-dependent DNA polymerase from human tissue culture cells (HeLa-fibroblast-synthetic oligonucleotides-template-purified enzymes).
MedLine Citation:
PMID:  4501125     Owner:  NLM     Status:  MEDLINE    
Two DNA polymerases that can copy synthetic RNA polymers are present in human tissue culture cells. These enzymes which have each been purified about 500-fold, are present in both HeLa cells, which are derived from a cervical carcinoma, and in WI-38 cells, a normal diploid strain originating from human embryonic lung tissue. These synthetic RNA-dependent DNA polymerases are identified by their ability to copy efficiently the ribo strand of synthetic oligonucleotide-homopolymer complexes, and differ in this respect from the known DNA-dependent DNA polymerases found in HeLa cells. The template requirements of these new DNA polymerases resemble that of the RNA-dependent DNA polymerases of the RNA tumor-viruses.
B Fridlender; M Fry; A Bolden; A Weissbach
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Feb 
Date Detail:
Created Date:  1972-04-26     Completed Date:  1972-04-26     Revised Date:  2010-09-13    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  452-5     Citation Subset:  IM    
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MeSH Terms
Cell Line
Cells, Cultured / analysis
Chromatography, DEAE-Cellulose
DNA / metabolism
DNA Nucleotidyltransferases / isolation & purification*
Hela Cells / drug effects,  enzymology*,  metabolism
Manganese / pharmacology
Protein Binding
RNA / metabolism*
Templates, Genetic
Reg. No./Substance:
0/Deoxyribonucleotides; 0/Polynucleotides; 10028-17-8/Tritium; 63231-63-0/RNA; 7439-96-5/Manganese; 9007-49-2/DNA; EC 2.7.7.-/DNA Nucleotidyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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