Document Detail


A new substrate for cultures of dissociated primary rat brain.
MedLine Citation:
PMID:  950025     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Different substrates were used to coat plastic petri dished for the cultivation of dissociated fetal rat brain cells. Only on surfaces which were coated with a mixture of serum and non-reconstituted collagen, did the majority of the inoculated cells attach singly or as aggregates within 24 h. The attachment of the cells was followed by the outgrowth of cellular processes either from single cells or from aggregates in the same time period. This did not occur on collagen or serum treated or on regular plastic dishes. Under the latter conditions a similar outgrowth was observed only after 3-5 days.
Authors:
R Mousson; R Maurer
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Experientia     Volume:  32     ISSN:  0014-4754     ISO Abbreviation:  Experientia     Publication Date:  1976 Jun 
Date Detail:
Created Date:  1976-10-02     Completed Date:  1976-10-02     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0376547     Medline TA:  Experientia     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  751-2     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / cytology*
Culture Media*
Culture Techniques / methods*
Fetus
Rats
Rats, Inbred F344
Chemical
Reg. No./Substance:
0/Culture Media

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