| A new strategy to induce effective antitumour response in vitro and in vivo. | |
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MedLine Citation:
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PMID: 18782257 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To induce Her2-specific cell immune response, we used xenogeneic antigen rat neu L2-S2 domains as the vaccine antigen. The antigenic protein was engineered as a chimeric protein with human IgG1 Fc region (neu-Fc). Neu-Fc could stimulate the cell proliferation in mixed lymphocyte reaction effectively. Simultaneous neu-Fc and IFN-gamma stimulation dramatically elevated IL-12 secretion and reduced IL-10 production in PBMC. To further augment the activating effects on Th1-type response, Bacille Calmette-Guerin (BCG) was utilized as a non-specific stimulus. Neu-Fc, IFN-gamma and BCG costimulation exhibited the most conspicuous effects on the reversal of the Th1-type inhibitory effects by MCF-7 cell supernatant compared with neu-Fc alone or IFN-gamma and BCG costimulation. The lytic activity of effector cells to Her2 overexpressing cells was greatly promoted by neu-Fc, IFN-gamma and BCG stimulation simultaneously. Neu-Fc led to considerable retardation in EMT6/Her2 tumour growth in Balb/c mice. IFN-gamma and BCG efficiently enhanced the antitumour activity. A large amount of inflammatory cells were found to be accumulated in the tumour tissues or surrounded tumours in mice treated with neu-Fc, IFN-gamma and BCG but no inflammatory cell infiltration was observed in control tumours, indicating that the strategy is potent enough to support the initiation and propagation of tumour-specific immune response in an established tumour and generate a proinflammatory environment. |
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Authors:
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M Wang; Z Xie; M Shi; H Lu; M Yu; M Hu; F Lu; Y Ma; B Shen; N Guo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Scandinavian journal of immunology Volume: 68 ISSN: 1365-3083 ISO Abbreviation: Scand. J. Immunol. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-10 Completed Date: 2008-10-07 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0323767 Medline TA: Scand J Immunol Country: England |
Other Details:
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Languages: eng Pagination: 287-96 Citation Subset: IM |
Affiliation:
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Institute of Basic Medical Sciences, Beijing, China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Heterophile / administration & dosage, therapeutic use* BCG Vaccine / administration & dosage, pharmacology*, therapeutic use* Cell Line, Tumor / metabolism Culture Media, Conditioned / pharmacology Cytotoxicity, Immunologic / drug effects* Female Glycoproteins / metabolism Histocompatibility Antigens Class I / metabolism Humans Inflammation / pathology Injections, Intravenous Injections, Subcutaneous Interferon-gamma / administration & dosage, pharmacology*, therapeutic use* Interleukin-10 / biosynthesis Interleukin-12 / antagonists & inhibitors*, metabolism Leukocytes, Mononuclear / drug effects, immunology, metabolism Mice Mice, Inbred BALB C Monocytes / drug effects*, immunology*, metabolism Neoplasms, Experimental / pathology, therapy Receptors, Fc / metabolism Recombinant Fusion Proteins / administration & dosage, pharmacology*, therapeutic use* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Heterophile; 0/BCG Vaccine; 0/Culture Media, Conditioned; 0/Erbb2 protein, rat; 0/Fc receptor, neonatal; 0/Glycoproteins; 0/Histocompatibility Antigens Class I; 0/Receptors, Fc; 0/Recombinant Fusion Proteins; 130068-27-8/Interleukin-10; 187348-17-0/Interleukin-12; 82115-62-6/Interferon-gamma |
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