Document Detail

A new stable and reliable method for labeling nucleic acids of fully replicative viruses.
MedLine Citation:
PMID:  22273842     Owner:  NLM     Status:  Publisher    
Efficiently labeling nucleic acids of fully replicative viruses is a challenge. In this work, a 'molecular light switch' complex [Ru(phen)(2)(dppz)](2+), where phen = 1,10-phenanthroline and dppz = dipyrido[3,2-a:2',3'-c]phenazine, has been exploringly used to label vaccinia virus nucleic acid. The labeled virions exhibited strong and stable fluorescence and could be imaged at the single-virion level. Moreover, they were fully infectious and can be used to study the behaviors of invasion into their host cells. The method is general and suitable for labeling various DNA viruses.
Li-Li Huang; Peng Zhou; Han-Zhong Wang; Rui Zhang; Jian Hao; Hai-Yan Xie; Zhi-Ke He
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-25
Journal Detail:
Title:  Chemical communications (Cambridge, England)     Volume:  -     ISSN:  1364-548X     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9610838     Medline TA:  Chem Commun (Camb)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
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