Document Detail


A new site-specific endonuclease, ScaI, from Streptomyces caespitosus.
MedLine Citation:
PMID:  2998344     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new site-specific endonuclease has been isolated from Streptomyces caespitosus and named ScaI. Based on analysis of sequences around the restriction sites in pBR322 and pBR325, the recognition sequence of ScaI endonuclease was deduced to be a new hexanucleotide 5'-AGTACT-3'. The cleavage site was determined by comparing the ScaI-cleaved product of a primer-extended M13mp18-SCA DNA, which contains an AGTACT sequence, with dideoxy chain terminator ladders of the same DNA. ScaI was found to cleave the recognition sequence between the internal T and A, leaving flush ends to the cleaved fragments.
Authors:
H Takahashi; H Kojima; H Saito
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  231     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1985 Oct 
Date Detail:
Created Date:  1985-12-04     Completed Date:  1985-12-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  229-32     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Binding Sites
DNA
DNA Restriction Enzymes / isolation & purification
Deoxyribonucleases, Type II Site-Specific*
Electrophoresis, Agar Gel
Streptomyces / enzymology*
Chemical
Reg. No./Substance:
9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/endodeoxyribonuclease ScaI; EC 3.1.21.4/Deoxyribonucleases, Type II Site-Specific
Comments/Corrections

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