Document Detail

A new sensitive fluorometric assay for the metabolism of (--)-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene by human hair follicles.
MedLine Citation:
PMID:  2265467     Owner:  NLM     Status:  MEDLINE    
A new sensitive fluorometric assay was established to measure the stereospecific formation of benzo[alpha]pyrene tetrols formed after cytochrome P450-dependent metabolism of (--)-7,8-dihydroxy-7,8- dihydrobenzo[a]pyrene by human hair follicles. This simple assay requires three human hair follicles and a low (0.5-2.0 microM) substrate concentration and has a limit of detection of approximately 0.3 fmol of tetrols. Freshly isolated human hair follicles from 20 adult volunteers (10 non-smokers and 10 smokers) were assayed. While intersubject and seasonal variations were observed, the assay was found to be reproducible for a given subject. This rapid and non-invasive assay provides a new means for metabolic phenotyping of human subjects for their capacity to metabolize (--)-7,8-dihydroxy-7,8-dihydrobenzo[alpha]pyrene to its carcinogenic form (+)anti-benzo[a]pyrene diolepoxide.
K Alexandrov; M Rojas; M Goldberg; A M Camus; H Bartsch
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carcinogenesis     Volume:  11     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1990 Dec 
Date Detail:
Created Date:  1991-02-12     Completed Date:  1991-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2157-61     Citation Subset:  IM    
Unit of Environmental Carcinogens and Host Factors, International Agency for Research on Cancer, Lyon, France.
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MeSH Terms
Benzo(a)pyrene / metabolism
Benzopyrenes / metabolism*,  pharmacology
Chromatography, High Pressure Liquid
Dose-Response Relationship, Drug
Fluorometry / methods
Hair / metabolism*
Molecular Conformation
Smoking / adverse effects
Time Factors
Reg. No./Substance:
0/Benzopyrenes; 36504-65-1/benzo(a)pyrene 7,8-oxide; 50-32-8/Benzo(a)pyrene

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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