| A new sensitive fluorometric assay for the metabolism of (--)-7,8-dihydroxy-7,8-dihydrobenzo[a]pyrene by human hair follicles. | |
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MedLine Citation:
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PMID: 2265467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A new sensitive fluorometric assay was established to measure the stereospecific formation of benzo[alpha]pyrene tetrols formed after cytochrome P450-dependent metabolism of (--)-7,8-dihydroxy-7,8- dihydrobenzo[a]pyrene by human hair follicles. This simple assay requires three human hair follicles and a low (0.5-2.0 microM) substrate concentration and has a limit of detection of approximately 0.3 fmol of tetrols. Freshly isolated human hair follicles from 20 adult volunteers (10 non-smokers and 10 smokers) were assayed. While intersubject and seasonal variations were observed, the assay was found to be reproducible for a given subject. This rapid and non-invasive assay provides a new means for metabolic phenotyping of human subjects for their capacity to metabolize (--)-7,8-dihydroxy-7,8-dihydrobenzo[alpha]pyrene to its carcinogenic form (+)anti-benzo[a]pyrene diolepoxide. |
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Authors:
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K Alexandrov; M Rojas; M Goldberg; A M Camus; H Bartsch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Carcinogenesis Volume: 11 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 1990 Dec |
Date Detail:
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Created Date: 1991-02-12 Completed Date: 1991-02-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2157-61 Citation Subset: IM |
Affiliation:
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Unit of Environmental Carcinogens and Host Factors, International Agency for Research on Cancer, Lyon, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Benzo(a)pyrene / metabolism Benzopyrenes / metabolism*, pharmacology Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Female Fluorometry / methods Hair / metabolism* Humans Molecular Conformation Smoking / adverse effects Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Benzopyrenes; 36504-65-1/benzo(a)pyrene 7,8-oxide; 50-32-8/Benzo(a)pyrene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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