Document Detail


A new and sensitive catalytic resonance scattering spectral assay for the detection of laccase using guaiacol as substrate.
MedLine Citation:
PMID:  21154922     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In pH 4.0 succinic acid-sodium hydroxide buffer solution, laccase catalyzed the oxidization of guaiacol substrate to form red particles, which exhibited a strong resonance scattering (RS) peak at 590 nm. Under the chosen conditions, as the laccase increased, the RS intensity (ΔI) increased linearly. The ΔI was proportional to laccase activity in the range of 0.10-1.2 U/mL, with a regression equation of ΔI = 734.0 U(laccase) - 9.7, and a detection limit of 0.05 U/mL. This RS method was applied to the detection of laccase activity in real samples, and the results were agreement with those from spectrophotometry. Copyright © 2010 John Wiley & Sons, Ltd.
Authors:
Caina Jiang; Shaoming Ling; Pengfei Wang; Aihui Liang; Bin Chen; Guiqing Wen; Zhiliang Jiang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2010-12-13
Journal Detail:
Title:  Luminescence : the journal of biological and chemical luminescence     Volume:  -     ISSN:  1522-7243     ISO Abbreviation:  -     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100889025     Medline TA:  Luminescence     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Ministry of Education, School of Environment and Resource, Guangxi Normal University, Guilin 541004, China.
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