Document Detail


A new respirometric endpoint-based biosensor to assess the relative toxicity of chemicals on immobilized human cells.
MedLine Citation:
PMID:  18499252     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several functional and biochemical parameters have been proposed as biomarkers of effect of environmental pollutants. A rapid biosensor working with immobilized human U-937 cells was developed and applied to environmentally relevant chemicals with different structures and toxicological pathways, i.e. benzalkonium chloride, clofibric acid, diclofenac, mercury nitrate, ofloxacin, and sodium dodecyl sulphate. Respiration of cells was relied upon as a comprehensive biochemical effect for screening purposes. Analytical parameter (DeltappmO(2)) and toxicological index (respiratory inhibition, delta%) measured after 1h of exposure were utilized for dose-response relationship study. Results (toxicity rating scales based on delta(50)% and steepness) were compared with those obtained by the same approach previously optimized on Saccharomyces cerevisiae. The toxicity rating scale obtained by the biomarker based on human mitochondrial and cell metabolic activities compared well with previous scale obtained on yeast cells and with available in-vivo acute toxicity indexes; respiration was confirmed as toxicological endpoint reliably measurable by the biosensor.
Authors:
Roberto Dragone; Chiara Frazzoli; Claudio Grappelli; Luigi Campanella
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-21
Journal Detail:
Title:  Ecotoxicology and environmental safety     Volume:  72     ISSN:  1090-2414     ISO Abbreviation:  Ecotoxicol. Environ. Saf.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-10-28     Completed Date:  2008-12-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7805381     Medline TA:  Ecotoxicol Environ Saf     Country:  United States    
Other Details:
Languages:  eng     Pagination:  273-9     Citation Subset:  IM    
Affiliation:
Institute for Complex Systems, Consiglio Nazionale delle Ricerche, Rome, Italy. roberto.dragone@uniroma1.it
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MeSH Terms
Descriptor/Qualifier:
Biological Assay
Biosensing Techniques / methods
Cells, Immobilized / drug effects*,  pathology
Diclofenac / toxicity
Dose-Response Relationship, Drug
Environmental Monitoring / methods*
Environmental Pollutants / toxicity*
Hazardous Substances / toxicity
Humans
Lethal Dose 50
Platinum / pharmacology
Saccharomyces cerevisiae / drug effects
U937 Cells / drug effects
Chemical
Reg. No./Substance:
0/Environmental Pollutants; 0/Hazardous Substances; 15307-86-5/Diclofenac; 7440-06-4/Platinum

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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