Document Detail

A new reference material for UV-visible circular dichroism spectroscopy.
MedLine Citation:
PMID:  18506835     Owner:  NLM     Status:  MEDLINE    
To obtain accurate and consistent measurements from circular dichroism (CD) instruments over time and from different laboratories, it is important that they are properly calibrated. The characteristics of the available reference materials are not ideal to ensure proper calibration as they typically only give peaks in one or two spectral regions, and often have issues concerning purity and stability. Currently either camphor sulfonic acid or ammonium camphor sulfonate are used. The latter can be an unstable, slightly hygroscopic secondary standard compound with only one characterized CD band. The former is the very hygroscopic primary standard for which only one enantiomer is readily available. We have synthesized a new reference material for CD, Na[Co(EDDS)].H(2)O (EDDS = N,N-ethylenediaminedisuccinic acid) which addresses these problems. It is extremely stable and available in both enantiomeric forms. The CD spectrum of Na[Co(EDDS)].H(2)O has nine distinct peaks between 180 and 599 nm. It thus fulfils the principal requirements for CD calibration chemical standards and has the potential to be used to ensure good practice in the measurement of CD data, providing two spectra of equal magnitude and opposite sign for a given concentration and path length. We have carried out an interlaboratory comparison using this material and show how it can be used to improve CD comparability between laboratories. A fitting algorithm has been developed to assess CD spectropolarimeter performance between 750 and 178 nm. This could be the basis of a formal quality control process once criteria for performance have been decided.
Angeliki Damianoglou; Edward J Crust; Matthew R Hicks; Suzanne E Howson; Alex E Knight; Jascindra Ravi; Peter Scott; Alison Rodger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chirality     Volume:  20     ISSN:  1520-636X     ISO Abbreviation:  Chirality     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-08-26     Completed Date:  2008-12-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8914261     Medline TA:  Chirality     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1029-38     Citation Subset:  IM    
Copyright Information:
(c) 2008 Wiley-Liss, Inc.
Department of Chemistry, University of Warwick, Coventry, United Kingdom.
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MeSH Terms
Circular Dichroism / methods*
Ethylenediamines / chemistry
Magnetic Resonance Spectroscopy
Models, Molecular
Molecular Structure
Spectrophotometry, Ultraviolet / methods*
Succinates / chemistry
Reg. No./Substance:
0/Ethylenediamines; 0/Succinates; 20846-91-7/N,N'-ethylenediamine disuccinic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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