Document Detail

A new protein conformation indicator based on biarsenical fluorescein with an extended benzoic acid moiety.
MedLine Citation:
PMID:  15055950     Owner:  NLM     Status:  MEDLINE    
We demonstrate herein a new protein conformation indicator based on biarsenical fluorescein with an extended benzoic acid moiety. The present indicator is reactive to a genetically introduced tetracysteine motif (Cys-Cys-Xaa-Xaa-Cys-Cys, where Xaa is a noncysteine amino acid) of proteins. Compared to the original biarsenical fluorescein (FlAsH) and the biarsenical Nile red analogue (BArNile), the present indicator exhibited larger fluorescence intensity changes in response to Ca(2+)-induced conformational rearrangements of calmodulin. A calculation of the highest occupied molecular orbital (HOMO) level of the benzoic acid moiety of the indicator molecule supports possible involvement of a photoinduced electron transfer (PET) process. These results indicate that the present indicator is useful for sensitive detection of protein conformational changes.
Jun Nakanishi; Mizuo Maeda; Yoshio Umezawa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  20     ISSN:  0910-6340     ISO Abbreviation:  Anal Sci     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-04-01     Completed Date:  2005-02-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  273-8     Citation Subset:  IM    
Bioengineering Laboratory, RIKEN, Hirosawa, Wako 351-0198, Japan.
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MeSH Terms
Benzoic Acid / chemistry*
Electrophoresis, Polyacrylamide Gel
Fluorescein / chemistry*
Fluorescent Dyes / chemistry
Protein Conformation*
Reg. No./Substance:
0/Fluorescent Dyes; 2321-07-5/Fluorescein; 65-85-0/Benzoic Acid

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