| A new process for simultaneous production of tannase and phytase by Paecilomyces variotii in solid-state fermentation of orange pomace. | |
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MedLine Citation:
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PMID: 21909682 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The production of enzymes such as tannases and phytases by solid-state fermentation and their use in animal feed have become a subject of great interest. In the present work, Paecilomyces variotii was used to produce tannase and phytase simultaneously. Solid-state fermentation, a process initially designed for tannase production, was implemented here using orange pomace as substrate. Orange pomace is the waste product of the large orange juice industry in Brazil, and it has also been used as an ingredient in animal feed. In addition to enzymatic production, biotransformation of the phenolic content and antioxidant capacity of the orange pomace were analyzed after fermentation. Fermentation conditions, namely moisture level and tannic acid concentration rate, were studied using CCD methodology. The response surface obtained indicated that the highest tannase activity was 5,000 U/gds after 96 h at 59% (v/w) and 3% (w/w) and that of phytase was 350 U/gds after 72 h at 66% (v/w) and 5.8% (w/w) of moisture level and tannic acid concentration, respectively. The amount of tannase production was similar to the levels achieved in previous studies, but this was accomplished with a 7% (w/w) reduction in the amount of supplemental tannic acid required. These results are the first to show that P. variotii is capable of producing phytase at significant levels. Moreover, the antioxidant capacity of orange pomace when tested against the free radical ABTS was increased by approximately tenfold as a result of the fermentation process. |
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Authors:
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Jose Valdo Madeira; Juliana Alves Macedo; Gabriela Alves Macedo |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-9-10 |
Journal Detail:
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Title: Bioprocess and biosystems engineering Volume: - ISSN: 1615-7605 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
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Created Date: 2011-9-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088505 Medline TA: Bioprocess Biosyst Eng Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Food Science Department, Faculty of Food Engineering, Campinas State University, P.O. Box 6121, CEP 13083-862, Sao Paulo, SP, Brazil, madeira_jr@hotmail.com. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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