| A new preparation method to study fresh plant structures with X-ray computed tomography. | |
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MedLine Citation:
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PMID: 19196405 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Since the development of X-ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good-quality images. Therefore, we present a new preparation method for fresh material which includes critical point drying and heavy metal staining. This technique enhances the contrast of fresh tissues, prevents artefacts such as tissue compression, and requires no embedding. |
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Authors:
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O Leroux; F Leroux; E Bellefroid; M Claeys; M Couvreur; G Borgonie; L Van Hoorebeke; B Masschaele; R Viane |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of microscopy Volume: 233 ISSN: 1365-2818 ISO Abbreviation: J Microsc Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2009-02-06 Completed Date: 2009-02-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0204522 Medline TA: J Microsc Country: England |
Other Details:
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Languages: eng Pagination: 1-4 Citation Subset: IM |
Affiliation:
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Research Group Pteridology, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Desiccation Plant Structures / ultrastructure* Specimen Handling / methods* Staining and Labeling / methods Tomography, X-Ray Computed* |
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