Document Detail


A new preparation method to study fresh plant structures with X-ray computed tomography.
MedLine Citation:
PMID:  19196405     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Since the development of X-ray computed tomography as a medical diagnostic tool, it was adapted and extended for many scientific applications, including plant structure research. As for many biological studies, sample preparation is of major importance to obtain good-quality images. Therefore, we present a new preparation method for fresh material which includes critical point drying and heavy metal staining. This technique enhances the contrast of fresh tissues, prevents artefacts such as tissue compression, and requires no embedding.
Authors:
O Leroux; F Leroux; E Bellefroid; M Claeys; M Couvreur; G Borgonie; L Van Hoorebeke; B Masschaele; R Viane
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of microscopy     Volume:  233     ISSN:  1365-2818     ISO Abbreviation:  J Microsc     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-02-06     Completed Date:  2009-02-27     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0204522     Medline TA:  J Microsc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1-4     Citation Subset:  IM    
Affiliation:
Research Group Pteridology, Department of Biology, Ghent University, K.L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Desiccation
Plant Structures / ultrastructure*
Specimen Handling / methods*
Staining and Labeling / methods
Tomography, X-Ray Computed*

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