Document Detail


A new polymorphic site in intron 2 of TP53 characterizes LOH in human tumors by PCR-SSCP.
MedLine Citation:
PMID:  7735557     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Many human cancers present deletions of the short arm of chromosome 17, which includes the TP53 locus. We detected a new polymorphism in intron 2 of the TP53 gene using PCR-SSCP and used this polymorphic site as a marker to detect loss of heterozygosity in 135 human tumors (73 soft tissue sarcomas, and 48 colorectal and 14 bladder carcinomas). Heterozygosity for this site was 41.5% in this study group and tumor-specific loss of alleles occurred in 43% of informative cases. Allelic losses were more frequently detected at this site than at that in which restriction fragment length polymorphism (RFLP) is located, as detected by the pHp53B probe. It is concluded that this novel approach has several advantages, including detection of a high incidence of informative cases and minimal tissue requirements.
Authors:
M R Oliva; G T Saez; E Latres; C Cordon-Cardo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Diagnostic molecular pathology : the American journal of surgical pathology, part B     Volume:  4     ISSN:  1052-9551     ISO Abbreviation:  Diagn. Mol. Pathol.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-06-07     Completed Date:  1995-06-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9204924     Medline TA:  Diagn Mol Pathol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  54-8     Citation Subset:  IM    
Affiliation:
Department of Pathology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Chromosome Deletion
Colorectal Neoplasms / genetics
Genes, p53 / genetics*
Heterozygote
Humans
Introns / genetics*
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic / genetics*
Polymorphism, Restriction Fragment Length
Polymorphism, Single-Stranded Conformational
Sarcoma / genetics
Soft Tissue Neoplasms / genetics
Urinary Bladder Neoplasms / genetics
Grant Support
ID/Acronym/Agency:
CA-47179/CA/NCI NIH HHS; CA-47538/CA/NCI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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