Document Detail

A new perspective on the inhibitory role of nitric oxide in sympathetic neurotransmission.
MedLine Citation:
PMID:  7488165     Owner:  NLM     Status:  MEDLINE    
By using, as a model of sympathetic neurons, immortalized rat pheochromocytoma (PC12) cells differentiated by nerve growth factor (NGF), the effect of nitric oxide on sympathetic neurotransmission was examined. The NO donor sodium nitroprusside (SNP; 10(-4)-3 x 10(-4) M) caused an apparent inhibition of dopamine release from PC12 cells, as measured by HPLC. Studies, in the absence of cells, involving the incubation of dopamine (20 ng/ml) or norepinephrine (15 ng/ml) with SNP (10(-6)-3 x 10(-4) M) or authentic NO (6 x 10(-6)-3 x 10(-5) M) revealed a similar reduction in the detection of the catecholamines. In addition, absorption spectroscopy studies showed dopamine and norepinephrine to be oxidized by NO resulting in the formation of their respective quinone products. These observations, coupled with the finding that the ability of dopamine to raise cAMP levels within PC12 cells was reduced after incubation with SNP, reveal that NO inhibits the biological activity rather than the release of catecholamines.
H Macarthur; M B Mattammal; T C Westfall
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  216     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1995 Nov 
Date Detail:
Created Date:  1995-12-21     Completed Date:  1995-12-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  686-92     Citation Subset:  IM    
Department of Pharmacological and Physiological Science, Saint Louis University School of Medicine, Missouri, USA.
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MeSH Terms
Adrenal Gland Neoplasms
Cell Differentiation / drug effects
Cyclic AMP / metabolism
Dopamine / metabolism,  pharmacology
Dose-Response Relationship, Drug
Drug Interactions
Nerve Growth Factors / pharmacology
Neurons / cytology,  drug effects,  physiology*
Nitric Oxide / pharmacology*,  physiology*
Nitroprusside / pharmacology*
Norepinephrine / metabolism,  pharmacology
PC12 Cells
Sympathetic Nervous System / physiology*
Synaptic Transmission / drug effects,  physiology*
Grant Support
Reg. No./Substance:
0/Nerve Growth Factors; 10102-43-9/Nitric Oxide; 15078-28-1/Nitroprusside; 51-41-2/Norepinephrine; 60-92-4/Cyclic AMP

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