Document Detail

A new pericentromeric repeated DNA sequence in Microtus thomasi.
MedLine Citation:
PMID:  19372666     Owner:  NLM     Status:  MEDLINE    
Several karyotypic forms have been previously described in populations of the vole species Microtus thomasi from Greece. In particular, the karyomorphs Microtus thomasi 'thomasi' and 'atticus' differ in X chromosome morphology, being acrocentric and subtelocentric, respectively. Furthermore, remarkable heterochromatin content variability has been described in sex chromosomes of both karyomorphs. Genomic DNA digestion with AluI allowed us to clone an 884 bp long repeated DNA sequence (Mth-Alu900) from the karyomorph M. thomasi 'atticus'. This repeated DNA is AT rich and seems to be organized mainly as a dimer of the 884-bp unit, which presents three simple repeats (CAAAT, CAGAT and CAGAC) that constitute 80% of the total unit length. This repeated DNA is exclusive to M. thomasi, since it is absent from the genome of other studied Arvicolinae species. The chromosomal location of Mth-Alu900 was analyzed on M. thomasi 'thomasi' and M. thomasi 'atticus' karyomorphs, with different sex chromosome constitution. It was mainly located on the pericentromeric heterochromatin of most autosomes and X chromosomes on both karyomorphs. Results are also discussed in relation to karyotypic and sex chromosome variations in M. thomasi. To our knowledge, Mth-Alu900 constitutes a new - the third discovered so far - pericentromeric repeated DNA sequence described in Microtus species.
M J Acosta; J A Marchal; G P Mitsainas; M T Rovatsos; C H Fernández-Espartero; E B Giagia-Athanasopoulou; A Sánchez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-15
Journal Detail:
Title:  Cytogenetic and genome research     Volume:  124     ISSN:  1424-859X     ISO Abbreviation:  Cytogenet. Genome Res.     Publication Date:  2009  
Date Detail:
Created Date:  2009-04-17     Completed Date:  2009-05-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101142708     Medline TA:  Cytogenet Genome Res     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  27-36     Citation Subset:  IM    
Copyright Information:
Copyright 2009 S. Karger AG, Basel.
Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain.
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MeSH Terms
Alu Elements
Arvicolinae / genetics
Base Sequence
Centromere / genetics
Chromosome Banding
DNA / genetics,  isolation & purification
In Situ Hybridization, Fluorescence
Molecular Sequence Data
Repetitive Sequences, Nucleic Acid / genetics*
Sequence Alignment
X Chromosome / genetics*
Y Chromosome / genetics*
Reg. No./Substance:

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