| A new organotypic model containing dermal-type macrophages. | |
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MedLine Citation:
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PMID: 22050324 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Human skin equivalents (SEs) are popular three-dimensional (D) cell culture systems in fundamental and applied dermatology. They have been made to contain dendritic cells, but so far no study on the incorporation of potentially anti-inflammatory dermal macrophages has been performed. Here, we show that monocyte-derived dermal-type macrophages can be introduced into a rigid scaffold with dermal fibroblasts. They maintain their cell surface markers CD163, DC-SIGN/CD209 and HLA-DR, which discriminate them from monocytes and dendritic cells. They retain the ability to produce the anti-inflammatory cytokine IL-10 in response to lipopolysaccharide (LPS) and to phagocytose latex beads. We thus demonstrate the feasibility of creating macrophage-fibroblast 3D cultures as a first step towards generating SEs with dermal macrophages. |
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Authors:
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Nicolas Bechetoille; Hortense Vachon; Amandine Gaydon; Aurelie Boher; Thomas Fontaine; Evelyne Schaeffer; Marion Decossas; Valérie André-Frei; Christopher G Mueller |
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Publication Detail:
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Type: LETTER Date: 2011-10-10 |
Journal Detail:
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Title: Experimental dermatology Volume: - ISSN: 1600-0625 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-11-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9301549 Medline TA: Exp Dermatol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 John Wiley & Sons A/S, Experimental Dermatology. |
Affiliation:
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BASF Beauty Care Solutions France SAS, Lyon, France CNRS UPR 9021, Laboratory of Therapeutic Immunology and Chemistry, IBMC, University of Strasbourg, Strasbourg, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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