| A new optical immunoassay for detection of S-100B protein in whole blood. | |
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MedLine Citation:
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PMID: 10617002 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: S-100B is a protein mainly found in astroglial cells and only detected to a low level in blood. Serum levels of S-100B increase in patients with acute brain injuries. The aim of this study was to establish feasibility of a new Optical ImmunoAssay ([OIA], Bio-Star, Inc, Boulder, CO) test for determination of S-100B in blood. METHODS: We have developed a new, rapid, and sensitive OIA test to identify elevated levels of S-100B in whole blood. The OIA test for S-100B combines monoclonal antibodies specific for the B-subunit of S-100 with OIA thin film technology. Each sample was tested for S-100B by the OIA method and a commercially available immunoluminometric assay. Blood samples were drawn serially from 9 patients undergoing coronary artery bypass graft surgery and during the early postoperative period. RESULTS: The OIA test determination of S-100B protein correlated with immunoluminometric assay data (r = 0.8) with a detection limit of 0.25 ng/mL. CONCLUSIONS: The sensitivity and feasibility of this rapid assay may be suitable for rapid evaluation of S-100B in urgent care settings (surgery, intensive care units, or emergency room). |
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Authors:
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A Ettinger; A B Laumark; R M Ostroff; J Brundell; W A Baumgartner; A Y Razumovsky |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Annals of thoracic surgery Volume: 68 ISSN: 0003-4975 ISO Abbreviation: Ann. Thorac. Surg. Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2000-01-13 Completed Date: 2000-01-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 15030100R Medline TA: Ann Thorac Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2196-201 Citation Subset: AIM; IM |
Affiliation:
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BioStar, Inc, Boulder, Colorado 80301, USA. a_ettinger@biostar.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aged Calcium-Binding Proteins / blood* Cerebrovascular Disorders / diagnosis, etiology Chemiluminescent Measurements Coronary Artery Bypass / adverse effects Feasibility Studies Female Humans Immunoassay / methods* Male Nerve Growth Factors / blood* S100 Proteins* Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Calcium-Binding Proteins; 0/Nerve Growth Factors; 0/S-100 calcium-binding protein beta subunit; 0/S100 Proteins |
| Comments/Corrections | |
Comment In:
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Ann Thorac Surg. 1999 Dec;68(6):2013-4
[PMID:
10616968
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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