Document Detail


A new non-functional form of milk xanthine oxidase containing stable quinquivalent molybdenum.
MedLine Citation:
PMID:  180983     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new non-functional modified form of milk xanthine oxidase is described. This contains molybdenum in a quinquivalent state, which is resistant to both oxidation and reduction. The new species is derived from the native enzyme in a two-step process. The first step is the conversion into the desulpho form, via loss of the 'persulphide' sulphur, and the second involves reaction with ethylene glycol or other reagents. The species gives a characteristic Mo(V) electron-paramagnetic-resonance signal, without proton splittings, designated Resting II. This is virtually identical with signals reported previously from resting turkey liver xanthine dehydrogenase and rabbit liver aldehyde oxidase. The possibility is discussed that species Resting II, prepared with ethylene glycol, contains a -COCH2OH residue bound to a nitrogen ligand of molybdenum.
Authors:
D J Lowe; M J Barber; R T Pawlik; R C Bray
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  155     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1976 Apr 
Date Detail:
Created Date:  1976-09-01     Completed Date:  1976-09-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  81-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aldehydes / pharmacology
Animals
Dioxins / pharmacology
Dithionite / pharmacology
Electron Spin Resonance Spectroscopy
Ethylene Glycols
Formaldehyde / pharmacology
Glycerol / pharmacology
Methanol / pharmacology
Milk / enzymology*
Molybdenum / analysis*
Temperature
Xanthine Oxidase / analysis*,  metabolism
Chemical
Reg. No./Substance:
0/Aldehydes; 0/Dioxins; 0/Ethylene Glycols; 14844-07-6/Dithionite; 50-00-0/Formaldehyde; 56-81-5/Glycerol; 67-56-1/Methanol; 7439-98-7/Molybdenum; EC 1.17.3.2/Xanthine Oxidase
Comments/Corrections

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