| A new multiplex real-time PCR test for HSV1/2 and syphilis: an evaluation of its impact in the laboratory and clinical setting. | |
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MedLine Citation:
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PMID: 20643660 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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OBJECTIVES: To develop, evaluate and implement a new multiplex real-time PCR test for the detection of herpes simplex virus (HSV)1, HSV2 and syphilis in a single sample using a single test. METHODS: A multiplex real-time PCR test detecting HSV1, HSV2 and Treponema pallidum was designed, validated and evaluated for a period of 6 months on patients attending the Sandyford Initiative (a series of genitourinary medicine clinics in and around Glasgow). A total of 692 samples were tested, and T pallidum PCR positives were confirmed by a second PCR at the Scottish Reference Laboratory (SBSTIRL). All PCR results were aligned with dark ground microscopy findings and serological results where available and compared. RESULTS: The laboratory validation of the multiplex assay showed the test to be sensitive, specific and robust. Of the 692 samples, 139 were positive for HSV1, 136 for HSV2, 15 for syphilis, one for both syphilis and HSV1, and 401 were negative; the reference laboratory confirmed all T pallidum PCR-positive samples. The PCR test was more sensitive than both dark ground microscopy and serological testing for the diagnosis of primary syphilis. CONCLUSIONS: The introduction of this new test has led to a better turnaround time for the diagnosis of genital ulcer disease, better detection of primary syphilis infection, and the detection of unexpected cases of syphilis where the aetiological agent suspected was HSV. |
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Authors:
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Laura Jane Scott; Rory N Gunson; William F Carman; Andrew J Winter |
Publication Detail:
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Type: Journal Article Date: 2010-07-18 |
Journal Detail:
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Title: Sexually transmitted infections Volume: 86 ISSN: 1472-3263 ISO Abbreviation: Sex Transm Infect Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9805554 Medline TA: Sex Transm Infect Country: England |
Other Details:
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Languages: eng Pagination: 537-9 Citation Subset: IM |
Affiliation:
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West of Scotland Specialist Virology Centre, Gartnavel General Hospital, 1053 Great Western Road, Glasgow G12OYN, UK. laura.jane.scott@ggc.scot.nhs.uk |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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