| A new model for studying the revascularization of skin grafts in vivo: the role of angiogenesis. | |
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MedLine Citation:
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PMID: 19050519 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Models of skin graft revascularization are based mostly on histologic evaluations but lack the possibility of analyzing the vascular biology in vivo. The aim of the present study was therefore to develop an animal model that allows continuous monitoring of the microcirculation during skin graft healing. METHODS: Skin and subcutaneous tissue were removed from the back of dorsal skinfold chamber preparations in mice, leaving one layer of striated muscle and subcutaneous tissue as a wound bed (n = 5). A corresponding full-thickness skin graft was harvested from the groin and sutured into the defect in the back of the chamber. To study graft healing, repetitive intravital microscopy was performed during the first 10 days after engraftment. RESULTS: Capillary widening in the wound bed appeared at day 1 after grafting and increased until day 4. Capillary buds and sprouts first appeared at day 2. Blood filling of autochthonous graft capillaries occurred at day 3, resulting in almost complete restoration of the original skin microcirculation on day 5. This was achieved by interconnections between the microvasculature of the wound bed and the skin graft through a temporary angiogenic response. In principle, angiogenic blood vessel growth originated in the wound bed and was directed toward the graft. CONCLUSIONS: This new model allows for repetitive analysis of the microcirculation during skin graft healing. It provides ideal in vivo conditions to further delineate the exact mechanisms of blood vessel interconnection during the complex process of angiogenesis, and may also allow study of the vascularization of tissue-engineered skin substitutes. |
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Authors:
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Nicole Lindenblatt; Maurizio Calcagni; Claudio Contaldo; Michael D Menger; Pietro Giovanoli; Brigitte Vollmar |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Plastic and reconstructive surgery Volume: 122 ISSN: 1529-4242 ISO Abbreviation: Plast. Reconstr. Surg. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-03 Completed Date: 2009-01-07 Revised Date: 2011-02-16 |
Medline Journal Info:
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Nlm Unique ID: 1306050 Medline TA: Plast Reconstr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 1669-80 Citation Subset: AIM; IM |
Affiliation:
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Division of Plastic and Reconstructive Surgery, University Hospital Zurich, Zurich, Switzerland. niclindenblatt@hotmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD31 / metabolism Bidens / metabolism Capillaries / physiology Dermatitis / physiopathology Hypoxia-Inducible Factor 1, alpha Subunit / metabolism Male Mice Mice, Inbred C57BL* Microcirculation / physiology Microscopy / instrumentation*, methods Models, Animal* Neovascularization, Physiologic / physiology* Skin / blood supply*, metabolism, surgery Skin Transplantation* Time Factors Tissue and Organ Harvesting Videotape Recording Wound Healing / physiology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD31; 0/Hif1a protein, mouse; 0/Hypoxia-Inducible Factor 1, alpha Subunit |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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