| A new model for the research into rhythmic contraction activity of cardiomyocytes in vitro. | |
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MedLine Citation:
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PMID: 8703415 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Heart cells continue to contract rhythmically after isolation and in culture in vitro. We describe a model of heart preparation in vitro that permits quantitative research on the frequency of contractions of cardiomyocytes. The chick embryo heart explants placed on a network of elastic glass fibers continued beating for months, recorded and analyzed with the methods of computer-assisted image analysis. The efficacy of this experimental model for the screening of effects of various agents on the frequency of contractions was examined by following the effects of nifedipine, caffeine, ethanol, and benzamide. The reversibility of the effects and the reproducibility of results were demonstrated quantitatively. The significance of a mechanical elastic load provided by glass fibers for the preservation of long-lasting contractile activity of cardiomyocytes is discussed and the common occurrence of oscillatory contraction processes in various eucaryotic cells is noted. |
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Authors:
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W Korohoda; A Jurkiewicz; I Figiel; J Czyz |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Biochemistry and cell biology = Biochimie et biologie cellulaire Volume: 73 ISSN: 0829-8211 ISO Abbreviation: Biochem. Cell Biol. Publication Date: 1995 Jul-Aug |
Date Detail:
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Created Date: 1996-09-06 Completed Date: 1996-09-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8606068 Medline TA: Biochem Cell Biol Country: CANADA |
Other Details:
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Languages: eng Pagination: 431-9 Citation Subset: IM; S |
Affiliation:
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Department of Cell Biology, Jagiellonian University, Kraków, Poland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzamides / pharmacology Caffeine / pharmacology Central Nervous System Agents / pharmacology Chick Embryo Drug Evaluation, Preclinical Ethanol / pharmacology Heart / drug effects, embryology, physiology* Models, Biological* Muscle Contraction / drug effects, physiology* Myocardium / cytology Periodicity* Reproducibility of Results |
| Chemical | |
Reg. No./Substance:
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0/Benzamides; 0/Central Nervous System Agents; 27208-38-4/benzamide; 58-08-2/Caffeine; 64-17-5/Ethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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