| A new model of focal cerebral ischemia in the miniature pig. | |
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MedLine Citation:
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PMID: 16506500 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECT: The purpose of this set of studies is to design a minimally invasive, reproducible stroke model in the gyrencephalic brain. This paper provides information on both surgical technique and methods of quantification of ischemic damage to both gray and white matter in the miniature pig. METHODS: Sixteen male miniature pigs were randomly divided into three groups and underwent transcranial surgery involving a frontotemporal approach with orbital rim osteotomy for permanent middle cerebral artery occlusion (MCAO; five animals), permanent internal carotid artery occlusion (ICAO; six animals), and a sham operation (five animals). Histological mapping and magnetic resonance (MR) imaging were used to delineate the areas of ischemic damage. The volumes of infarction measured directly from MR images were 16.2 +/- 1.1, 1.5 +/- 0.5, and 0.0 +/- 0.0 cm3 (mean +/- standard deviation [SD], p < 0.001) in the MCAO, ICAO, and sham-operated groups, respectively. The areas of ischemia identified through histological analysis and MR imaging showed a good correlation (r2 = 0.86, p < 0.0001). Immunohistochemical staining with an amyloid precursor protein (APP) antibody was used to evaluate axonal damage and calculate a total APP score for axonal damage of 44.8 +/- 2.9 in the MCAO, 13.2 +/- 6.6 in the ICAO, and 0.0 +/- 0.0 (mean +/- SD, p < 0.002) in the sham-operated animals. CONCLUSIONS: This new model of focal cerebral ischemia induces a reproducible amount of ischemic damage in both gray and white matter, and has significant utility for studies of the pathophysiology of ischemia in the gyrencephalic brain and for assessment of the therapeutic efficacy of drugs prior to the initiation of human clinical trials. |
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Authors:
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Hideaki Imai; Kenjiro Konno; Mitsunobu Nakamura; Tatsuya Shimizu; Chisato Kubota; Koji Seki; Fumiaki Honda; Shinichiro Tomizawa; Yukitaka Tanaka; Hidekazu Hata; Nobuhito Saito |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of neurosurgery Volume: 104 ISSN: 0022-3085 ISO Abbreviation: J. Neurosurg. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-03-01 Completed Date: 2006-03-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0253357 Medline TA: J Neurosurg Country: United States |
Other Details:
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Languages: eng Pagination: 123-32 Citation Subset: AIM; IM |
Affiliation:
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Department of Neurosurgery, Institute of Experimental Animal Research, Gunma University Graduate School of Medicine, Maebashi, Gunma, Japan. hideimai@showa.gunma-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Ischemia / physiopathology*, veterinary Disease Models, Animal* Immunohistochemistry Magnetic Resonance Imaging Male Middle Cerebral Artery / pathology, surgery Osteotomy Random Allocation Reproducibility of Results Swine Swine, Miniature |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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